Integrated application of transcriptomics and metabolomics provides insights into acute hepatopancreatic necrosis disease resistance of Pacific white shrimp Litopenaeus vannamei

doi:10.1128/msystems.00067-23

IOCAS-IR > 实验海洋生物学重点实验室

	Integrated application of transcriptomics and metabolomics provides insights into acute hepatopancreatic necrosis disease resistance of Pacific white shrimp Litopenaeus vannamei
	Sun, Mingzhe1,2,3 ; Yu, Yang1,2,3,4 ; Li, Shihao 1,2,3,4; Liu, Yuan 1,2,3,4; Zhang, Xiaojun 1,2,3,4; Li, Fuhua 1,2,3,4,5
	2023-06-26
发表期刊	MSYSTEMS
ISSN	2379-5077
页码	20
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
摘要	Acute hepatopancreatic necrosis disease (AHPND) has caused a huge economic loss to shrimp aquaculture. Vibrio parahaemolyticus (Vp(AHPND)) is regarded as a major causative agent of AHPND in the Pacific white shrimp Litopenaeus vannamei. However, knowledge about how shrimp resist to AHPND is very limited. In order to learn the molecular mechanisms underlying AHPND resistance of shrimp, comparison between disease-resistant family and susceptible family of L. vannamei were performed at transcriptional and metabolic levels. Integrated analysis of transcriptomics and metabolomics on hepatopancreas of shrimp, the target tissue of Vp(AHPND), showed that significant differences existed between resistant family and susceptible family of shrimp. The susceptible family showed higher level of glycolysis, serine-glycine metabolism, purine and pyrimidine metabolism, but lower level of betaine-homocysteine metabolism in the hepatopancreas in comparison with the resistant family without Vp(AHPND) infection. Curiously, Vp(AHPND) infection induced up-regulation of glycolysis, serine-glycine metabolism, purine metabolism, pyrimidine metabolism, and pentose phosphate pathway, and down-regulation of betaine-homocysteine metabolism in resistant family. In addition, arachidonic acid metabolism and some immune pathways, like NF-& kappa;B and cAMP pathways, were up-regulated in the resistant family after Vp(AHPND) infection. In contrast, amino acid catabolism boosted via PEPCK-mediated TCA cycle flux was activated in the susceptible family after Vp(AHPND) infection. These differences in transcriptome and metabolome between resistant family and susceptible family might contribute to the resistance of shrimp to bacteria. IMPORTANCEVibrio parahaemolyticus (Vp(AHPND)) is a major aquatic pathogen causing acute hepatopancreatic necrosis disease (AHPND) and leads to a huge economic loss to shrimp aquaculture. Despite the recent development of controlling culture environment, disease resistant broodstock breeding is still a sustainable approach for aquatic disease control. Metabolic changes occurred during Vp(AHPND) infection, but knowledge about the metabolism in resistance to AHPND is very limited. Integrated analysis of transcriptome and metabolome revealed the basal metabolic differences exhibited between disease-resistant and susceptible shrimp. Amino acid catabolism might contribute to the pathogenesis of Vp(AHPND) and arachidonic acid metabolism might be responsible for the resistance phenotype. This study will help to enlighten the metabolic and molecular mechanisms underlying shrimp resistance to AHPND. Also, the key genes and metabolites of amino acid and arachidonic acid pathway identified in this study will be applied for disease resistance improvement in the shrimp culture industry. Vibrio parahaemolyticus (Vp(AHPND)) is a major aquatic pathogen causing acute hepatopancreatic necrosis disease (AHPND) and leads to a huge economic loss to shrimp aquaculture. Despite the recent development of controlling culture environment, disease resistant broodstock breeding is still a sustainable approach for aquatic disease control. Metabolic changes occurred during Vp(AHPND) infection, but knowledge about the metabolism in resistance to AHPND is very limited. Integrated analysis of transcriptome and metabolome revealed the basal metabolic differences exhibited between disease-resistant and susceptible shrimp. Amino acid catabolism might contribute to the pathogenesis of Vp(AHPND) and arachidonic acid metabolism might be responsible for the resistance phenotype. This study will help to enlighten the metabolic and molecular mechanisms underlying shrimp resistance to AHPND. Also, the key genes and metabolites of amino acid and arachidonic acid pathway identified in this study will be applied for disease resistance improvement in the shrimp culture industry.
关键词	Litopenaeus vannamei acute hepatopancreatic necrosis disease disease resistance transcriptome metabolome
DOI	10.1128/msystems.00067-23
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[32102830] ; ~National Natural Science Foundation of China (NSFC)[31972829] ; ~National Natural Science Foundation of China (NSFC)[31830100] ; earmarked fund for CARS-48 ; Taishan Scholar Project of Shandong Province
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001026304000001
出版者	AMER SOC MICROBIOLOGY
WOS关键词	ONE-CARBON METABOLISM ; VIBRIO-PARAHAEMOLYTICUS ; ARACHIDONIC-ACID ; PENAEID SHRIMP ; ROLES ; AHPND ; GLUCONEOGENESIS ; METHYLATION ; EXPRESSION ; PEPCK
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182512
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Sun, Mingzhe,Yu, Yang,Li, Shihao,et al. Integrated application of transcriptomics and metabolomics provides insights into acute hepatopancreatic necrosis disease resistance of Pacific white shrimp Litopenaeus vannamei[J]. MSYSTEMS,2023:20.
APA	Sun, Mingzhe,Yu, Yang,Li, Shihao,Liu, Yuan,Zhang, Xiaojun,&Li, Fuhua.(2023).Integrated application of transcriptomics and metabolomics provides insights into acute hepatopancreatic necrosis disease resistance of Pacific white shrimp Litopenaeus vannamei.MSYSTEMS,20.
MLA	Sun, Mingzhe,et al."Integrated application of transcriptomics and metabolomics provides insights into acute hepatopancreatic necrosis disease resistance of Pacific white shrimp Litopenaeus vannamei".MSYSTEMS (2023):20.