A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

doi:10.3390/ijms241310665

IOCAS-IR > 实验海洋生物学重点实验室

	A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation
	Guo, Na 1,2; Liu, Yuan 2,3; Hao, Qiang 1,2; Sun, Mingzhe2,3 ; Li, Fuhua 2,3,4
	2023-07-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	24 期号:13 页码:19
通讯作者	Liu, Yuan(liuyuan@qdio.ac.cn)
摘要	Mannose receptor, as a member of the C-type lectin superfamily, is a non-canonical pattern recognition receptor that can internalize pathogen-associated ligands and activate intracellular signaling. Here, a mannose receptor gene, LvMR, was identified from the Pacific white shrimp Litopenaeus vannamei. LvMR encoded a signal peptide, a fibronectin type II (FN II) domain, and two carbohydrate-recognition domains (CRDs) with special EPS and FND motifs. LvMR transcripts were mainly detected in the hepatopancreas, and presented a time-dependent response after pathogen challenge. The recombinant LvMR (rLvMR) could bind to various PAMPs and agglutinate microorganisms in a Ca2+-dependent manner with strong binding ability to (D)-mannose and N-acetyl sugars. The knockdown of LvMR enhanced the expression of most NF-& kappa;B pathway genes, inflammation and redox genes, while it had no obvious effect on the transcription of most phagocytosis genes. Moreover, the knockdown of LvMR caused an increase in reactive oxygen species (ROS) content and inducible nitric oxide synthase (iNOS) activity in the hepatopancreas after Vibrio parahaemolyticus infection. All these results indicate that LvMR might perform as a PRR in immune recognition and a negative regulator of inflammation during bacterial infection.
关键词	mannose receptor Litopenaeus vannamei pathogen recognition inflammation ROS
DOI	10.3390/ijms241310665
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[31830100] ; National Natural Science Foundations of China[32273172] ; Taishan Scholars Program ; [CARS-48]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001030955700001
出版者	MDPI
WOS关键词	C-TYPE-LECTIN ; MOLECULAR-CLONING ; SHRIMP ; IDENTIFICATION ; HEMOCYTES ; CRD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182500
专题	实验海洋生物学重点实验室
通讯作者	Liu, Yuan
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Guo, Na,Liu, Yuan,Hao, Qiang,et al. A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(13):19.
APA	Guo, Na,Liu, Yuan,Hao, Qiang,Sun, Mingzhe,&Li, Fuhua.(2023).A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(13),19.
MLA	Guo, Na,et al."A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.13(2023):19.