Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences

doi:10.1016/j.gene.2023.147534

IOCAS-IR

	Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences
	Cui, Xiaodong 1; Yang, Mei 2,3; Li, Chunyu 1; An, Bo 1; Mu, Shumei 1; Zhang, Han 1; Chen, Yongxia 1; Li, Xinzheng 2,3,4,5; Kang, Xianjiang 1
	2023-08-15
发表期刊	GENE
ISSN	0378-1119
卷号	877 页码:10
通讯作者	Li, Xinzheng(lixzh@qdio.ac.cn) ; Kang, Xianjiang(xjkang@hbu.edu.cn)
摘要	Neocaridina denticulata sinensis is a crustacean of major economic significance in the Baiyangdian drainage area. In this study, the first assessment of N. denticulata sinensis genetic diversity and population structure was performed based on sequence analysis of nine polymorphic microsatellite loci and the mitochondrial cytochrome oxidase subunit I (cox1) gene. Samples (n = 192) were collected from four different regions in the Baiyangdian drainage area i.e., Baiyangdian Lake, Jumahe River, Xidayang Reservoir, and Fuhe River. Microsatellite loci analysis identified high levels of genetic diversity represented by observed heterozygosity (Ho) of 0.6865 similar to 0.9583, expected heterozygosity (He) of 0.7151 similar to 0.8723, and polymorphism information content (PIC) of 0.6676 similar to 0.8585. Based on the analysis of cox1 sequences, haplotype diversity (Hd) ranged from 0.568 to 0.853 while nucleotide diversity (pi) ranged from 0.0029 to 0.2236. Furthermore, there was no evidence of expansion events in the N. denticulata sinensis populations. Pairwise FST revealed pronounced genetic differentiation, and clustering analyses showed defined genetic structures within the N. denticulata sinensis population. Three groups were identified from four sampled stocks, with Xidayang Reservoir, and Fuhe River populations clustered in the same group. This work identified novel molecular markers and provided an important reference to guide management strategies to assist conservation of N. denticulata sinensis resources.
关键词	Genetic differentiation Population structure Microsatellite Mitochondrial DNA Neocaridina denticulata sinensis
DOI	10.1016/j.gene.2023.147534
收录类别	SCI
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001019961300001
出版者	ELSEVIER
WOS关键词	SHRIMP PENAEUS-MONODON ; WHITE SHRIMP ; MOLECULAR DIVERSITY ; DNA ; DECAPODA ; DIFFERENTIATION ; POLYMORPHISM ; ATYIDAE ; RIVER ; SEA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182473
专题	中国科学院海洋研究所
通讯作者	Li, Xinzheng; Kang, Xianjiang
作者单位	1.Hebei Univ, Coll Life Sci, Baoding, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cui, Xiaodong,Yang, Mei,Li, Chunyu,et al. Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences[J]. GENE,2023,877:10.
APA	Cui, Xiaodong.,Yang, Mei.,Li, Chunyu.,An, Bo.,Mu, Shumei.,...&Kang, Xianjiang.(2023).Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences.GENE,877,10.
MLA	Cui, Xiaodong,et al."Assessment of genetic diversity and population structure of Neocaridina denticulata sinensis in the Baiyangdian drainage area, China, using microsatellite markers and mitochondrial cox1 gene sequences".GENE 877(2023):10.