Tea polyphenols, astaxanthin, and melittin can significantly enhance the immune response of juvenile spotted knifejaw (Oplegnathus punctatus)

doi:10.1016/j.fsi.2023.108817

IOCAS-IR > 实验海洋生物学重点实验室

	Tea polyphenols, astaxanthin, and melittin can significantly enhance the immune response of juvenile spotted knifejaw (Oplegnathus punctatus)
	Wu, Yanduo 1,2,3; Xiao, Yongshuang 1,2; Li, Wensheng 4; Yang, Chuanjun 4; Ma, Wenhui 4; Pang, Zunfang 4; Zhang, Jiawei 4; Xiao, Zhizhong 1,2; Li, Jun 1,2
	2023-07-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	138 页码:6
通讯作者	Xiao, Zhizhong(xzz@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	The frequent occurrence of diseases seriously hampers the sustainable development of the spotted knifejaw (Oplegnathus punctatus) breeding industry. Our previous genome-wide scan and cross-species comparative genomic analysis revealed that the immune gene family (Toll-like receptors, TLR) members of O. punctatus underwent a significant contraction event (tlr1, tlr2, tlr14, tlr5, and tlr23). To address immune genetic contraction may result in reduced immunity, we investigated whether adding different doses (0, 200, 400, 600, and 800 mg/ kg) of immune enhancers (tea polyphenols, astaxanthin, and melittin) to the bait after 30 days of continuous feeding could stimulate the immune response of O. punctatus. We found that the expression of tlr1, tlr14, tlr23 genes in immune organs (spleen and head kidney) was stimulated when tea polyphenols were added at 600 mg/ kg. The tlr2 (400 mg/kg), tlr14 (200 mg/kg), tlr5 (200 mg/kg), and tlr23 (200 mg/kg) genes expression of in-testine were elevated in the tea polyphenol group. When the addition of astaxanthin is 600 mg/kg, it can effectively stimulate the expression of tlr14 gene in immune organs (liver, spleen and head kidney). In the astaxanthin group, the expression of the genes tlr1 (400 mg/kg), tlr14 (600 mg/kg), tlr5 (400 mg/kg) and tlr23 (400 mg/kg) reached their highest expression in the intestine. Besides, the addition of 400 mg/kg of melittin can effectively induce the expression of tlr genes in the liver, spleen and head kidney, except the tlr5 gene. The tlr- related genes expression in the intestine was not significantly elevated in the melittin group. We hypothesize that the immune enhancers could enhance the immunity of O. punctatus by increasing the expression of tlr genes, and thereby leading to increased resistance to diseases. Meanwhile, our findings further demonstrated that significant increases in weight gain rate (WGR), visceral index (VSI), and feed conversion rate (FCR) were observed at 400 mg/kg, 200 mg/kg and 200 mg/kg of tea polyphenols, astaxanthin and melittin in the diet, respectively. Overall, our study provided valuable insights for future immunity enhancement and viral infection prevention in O. punctatus, as well as offered guidance for the healthy development of the O. punctatus breeding industry.
关键词	Feed additives Toll-like receptors Immune response Growth performance Oplegnathus punctatus
DOI	10.1016/j.fsi.2023.108817
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2022YFC3103600] ; National Key Research and Development Program[2022YFC3102004] ; National Natural Science Foundation of China[42276107] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[CARS-47] ; CARs for Marine Fish Culture Industry ; [COMS2020Q05]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001013393000001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS关键词	GROWTH-PERFORMANCE ; SUPPLEMENTATION ; GENES ; VENOM
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182365
专题	实验海洋生物学重点实验室
通讯作者	Xiao, Zhizhong; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Laizhou Mingbo Aquat Prod Co Ltd, Yantai 261418, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Yanduo,Xiao, Yongshuang,Li, Wensheng,et al. Tea polyphenols, astaxanthin, and melittin can significantly enhance the immune response of juvenile spotted knifejaw (Oplegnathus punctatus)[J]. FISH & SHELLFISH IMMUNOLOGY,2023,138:6.
APA	Wu, Yanduo.,Xiao, Yongshuang.,Li, Wensheng.,Yang, Chuanjun.,Ma, Wenhui.,...&Li, Jun.(2023).Tea polyphenols, astaxanthin, and melittin can significantly enhance the immune response of juvenile spotted knifejaw (Oplegnathus punctatus).FISH & SHELLFISH IMMUNOLOGY,138,6.
MLA	Wu, Yanduo,et al."Tea polyphenols, astaxanthin, and melittin can significantly enhance the immune response of juvenile spotted knifejaw (Oplegnathus punctatus)".FISH & SHELLFISH IMMUNOLOGY 138(2023):6.