Insights into the fatty acid binding protein of Pacific white shrimp (Litopenaeus vannamei) suffering acute hepatopancreatic necrosis disease infection

doi:10.1016/j.aquaculture.2023.739719

IOCAS-IR > 实验海洋生物学重点实验室

	Insights into the fatty acid binding protein of Pacific white shrimp (Litopenaeus vannamei) suffering acute hepatopancreatic necrosis disease infection
	Gu, Xiaoqian 1,2,4; Wang, Baojie 1,2; Jiang, Keyong 1,2; Liu, Mei 3,5; Wang, Lei 1,2,4,6
	2023-09-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	574 页码:12
通讯作者	Liu, Mei(mliu_msrisd@126.com) ; Wang, Lei(leiwang@qdio.ac.cn)
摘要	Acute hepatopancreatic necrosis disease (AHPND) has a significant economic impact on aquaculture worldwide. However, the molecular mechanism responsible for AHPND remains unknown. Our previous study demonstrated that the Litopenaeus vannamei fatty acid binding protein (LvFABP) interacted with the Vibrio parahaemolyticus toxin PirB, and that silencing LvFABP using RNA interference improved AHPND resistance in shrimp. Polyclonal antibodies against recombinant LvFABP and PirBvp toxin were produced to further elucidate the role of LvFABP in AHPND resistance. Immunofluorescence localization showed that LvFABP was abundant throughout the cytoplasm of the hepatopancreas cells, and a co-localization assay revealed that PirBvp directly interacted with LvFABP. Antibodies against LvFABP were able to partially block the binding of LvFABP to PirBvp in vitro and inhibited AHPND infection in vivo. Exogenous recombinant LvFABP had a partially neutralizing effect against AHPND and gave L. vannamei significant protection against infection. In addition, recombinant LvFABP treatment of L. vannamei significantly reduced the morphological damage to the shrimp following pathogen challenge. The transcriptomes of the L. vannamei hepatopancreas with and without V. parahaemolyticus infection after silencing of LvFABP by RNA interference were also explored. The results showed that AHPND infection could affect the health of L. vannamei via functional changes in carbohydrate metabolism, lipid metabolism, and transport and catabolism. Furthermore, silencing LvFABP could effectively protect metabolism, transport and catabolic homeostasis, thus enabling adaptation to further or prolonged AHPND infections. These findings provided a unique and important dataset that deepened our understanding of the pathological mechanism of AHPND and may help in the development of strategies to prevent and ameliorate the effects of bacterial diseases.
关键词	Fatty acid binding protein AHPND Immunofluorescence localization Blocking assay Neutralization assay Transcriptome analysis
DOI	10.1016/j.aquaculture.2023.739719
收录类别	SCI
语种	英语
资助项目	Yellow River Delta Industry Leading Talent Project ; National Key Ramp;D Program of China[2019YFD0900401]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001012431100001
出版者	ELSEVIER
WOS关键词	DIFFERENTIALLY EXPRESSED GENES ; SPOT SYNDROME VIRUS ; VIBRIO-PARAHAEMOLYTICUS ; TRANSCRIPTOME ANALYSIS ; PPAR-ALPHA ; AHPND ; DEFICIENT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182340
专题	实验海洋生物学重点实验室
通讯作者	Liu, Mei; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Marine Sci Res Inst Shandong Prov, Shandong Key Lab Dis Control Mariculture, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Marine Sci Res Inst Shandong Prov, 7 Youyun Rd, Qingdao 266104, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gu, Xiaoqian,Wang, Baojie,Jiang, Keyong,et al. Insights into the fatty acid binding protein of Pacific white shrimp (Litopenaeus vannamei) suffering acute hepatopancreatic necrosis disease infection[J]. AQUACULTURE,2023,574:12.
APA	Gu, Xiaoqian,Wang, Baojie,Jiang, Keyong,Liu, Mei,&Wang, Lei.(2023).Insights into the fatty acid binding protein of Pacific white shrimp (Litopenaeus vannamei) suffering acute hepatopancreatic necrosis disease infection.AQUACULTURE,574,12.
MLA	Gu, Xiaoqian,et al."Insights into the fatty acid binding protein of Pacific white shrimp (Litopenaeus vannamei) suffering acute hepatopancreatic necrosis disease infection".AQUACULTURE 574(2023):12.