Characterization of nongxiang- and soy sauce-type distilled grains and their application in enhancing polyunsaturated fatty acid and triacylglyceride production from Chlorella sp. 038F

doi:10.1016/j.jece.2023.110174

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of nongxiang- and soy sauce-type distilled grains and their application in enhancing polyunsaturated fatty acid and triacylglyceride production from Chlorella sp. 038F
	Yang, Na 1,2; Shangguan, Jingjing 1,3; Zhang, Litao 1,2; Li, Yueming 4; Xu, Jianchun 4; Xia, Xiuluan 4; Liu, Jianguo 1,2
	2023-06-01
发表期刊	JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
ISSN	2213-2929
卷号	11 期号:3 页码:9
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	A sustainable method of handling large quantities of distilled grains in the Chinese baijiu industry is bioconverting these distilled grains into value-added products. We here compared the properties of two distilled grains, soy sauce-type (DGSB) and nongxiang-type (DGNB). Results revealed that DGSB had higher contents of crude protein, crude fat, soluble sugars, free amino acids, and antioxidants than DGNB. Mixotrophic cultivation of Chlorella sp. 038F was performed using aqueous crude extracts of distilled grains. Biomass, total lipid, and fatty acid production with DGSB crude extracts was approximately 2.43, 1.40, and 1.69 times higher than that with photoautotrophic culture, respectively, whereas DGNB crude extracts only improved the biomass by 1.64 times. Detailed profile analysis revealed that DGSB crude extracts remarkably increased the proportions of polyunsaturated fatty acids, especially linoleic and & alpha;-linolenic acids, and triacylglycerides in total lipids. Interestingly, DGNB crude extracts did not enhance total lipid accumulation but increased triacylglyceride accumulation. Comparative transcriptomics revealed that genes encoding photosynthetic pigments and proteins were downregulated, suggesting the decreased anabolism of the photosynthetic light reaction. Genes involved in fatty acid biosynthesis, the Calvin cycle, and glycolysis were upregulated, indicating sufficient accumulation of the building blocks acyl-CoA and glycerol-3-phosphate for triacylglyceride biosynthesis. All transcriptional trends of triacylglyceride biosynthesis-related genes were consistent with the triacylglyceride accumulation patterns. This study provides insights into the potential use of microalgae for the bioconversion of distilled grains into highvalue products and highlights the role of distilled grains in improving polyunsaturated fatty acid and triacylglyceride production.
关键词	Distilled grains Chlorella sp 038F Mixotrophic cultivation Polyunsaturated fatty acid Triacylglyceride
DOI	10.1016/j.jece.2023.110174
收录类别	SCI
语种	英语
资助项目	TaiShan Industrial Experts Programme of the Chinese Academy of Sciences, China[tscy20200102] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000]
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:001018618100001
出版者	ELSEVIER SCI LTD
WOS关键词	MIXOTROPHIC CULTIVATION ; CHINESE SPIRIT ; WASTE ; FERMENTATION ; PYRENOIDOSA ; FEEDSTOCK ; BIOFUEL ; GROWTH
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182334
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Langyatai Grp Co Ltd, Qingdao 266500, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Na,Shangguan, Jingjing,Zhang, Litao,et al. Characterization of nongxiang- and soy sauce-type distilled grains and their application in enhancing polyunsaturated fatty acid and triacylglyceride production from Chlorella sp. 038F[J]. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING,2023,11(3):9.
APA	Yang, Na.,Shangguan, Jingjing.,Zhang, Litao.,Li, Yueming.,Xu, Jianchun.,...&Liu, Jianguo.(2023).Characterization of nongxiang- and soy sauce-type distilled grains and their application in enhancing polyunsaturated fatty acid and triacylglyceride production from Chlorella sp. 038F.JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING,11(3),9.
MLA	Yang, Na,et al."Characterization of nongxiang- and soy sauce-type distilled grains and their application in enhancing polyunsaturated fatty acid and triacylglyceride production from Chlorella sp. 038F".JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 11.3(2023):9.