Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation

doi:10.1007/s10811-023-03008-w

IOCAS-IR > 实验海洋生物学重点实验室

	Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation
	Jia, Shuao 1,2,3,4; Wu, Songcui1,2,3 ; Liu, Xuehua 1,2,3; Gu, Wenhui 1,2,3; Wang, Guangce 1,2,3
	2023-06-10
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
页码	16
通讯作者	Gu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	The halotolerant green alga Dunaliella salina is widely cultivated in the industrial-scale production of natural beta-carotene (containing 9-cis and all-trans isomers) due to its high carotenoid content. The management of environmental factors such as nitrogen deficiency is an efficient way to induce the production of beta-carotene. However, it is not clear how changes in nitrogen concentration and the resulting changes in the carbon-nitrogen (C/N) ratio affect the beta-carotene isomer configuration ratio and content. In this study, D. salina HG01 was cultured under different C/N ratios, growth parameters, beta-carotene content and the ratio of two isomers, intracellular substance content, and antioxidant enzyme activity were determined. The results showed that although a large amount of beta-carotene was accumulated in algae cells under a high C/N ratio, the high C/N ratio was not conducive to the increase of beta-carotene content in unit culture volume and the proportion of 9-cis-beta-carotene. However, the beta-carotene content in per unit of culture volume (14.70 mu g mL(-1)) under a medium C/N ratio and the proportion of 9-cis-beta-carotene (39.31%) under a low C/N ratio were relatively higher. In addition, transcriptome sequencing revealed changes in the expression of genes related to photosynthesis, carbon and nitrogen. This study integrates the physiological and transcriptomics data related with beta-carotene configuration changes in D. salina under different C/N ratios, which has practical implications for the industrial production of beta-carotene. By optimizing the C/N ratio during cultivation, it is possible to enhance beta-carotene content and improve the desired 9-cis isomer proportion. These findings can contribute to the development of more efficient and cost-effective production methods for natural beta-carotene, thereby benefiting D. salinabased beta-carotene industry.
关键词	Dunaliella salina Carbon-nitrogen ratio (C/N) 9-cis beta-carotene Carotenoids biosynthesis Metabolic regulation
DOI	10.1007/s10811-023-03008-w
收录类别	SCI
语种	英语
资助项目	Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; National Natural Science Foundation of China[42206097] ; National Natural Science Foundation of China[42276146] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Marine Bio-logical Carbon Cycling Research ; Chinese Academy of Sciences[COMS2019Q02]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:001006605400001
出版者	SPRINGER
WOS关键词	BETA-CAROTENE ; GENETIC-TRANSFORMATION ; TRANSCRIPTOME ANALYSIS ; ANTIOXIDANT ACTIVITY ; ALL-TRANS ; ALGA ; BARDAWIL ; BIOTECHNOLOGY ; DWARF27 ; MODE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182312
专题	实验海洋生物学重点实验室
通讯作者	Gu, Wenhui; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jia, Shuao,Wu, Songcui,Liu, Xuehua,et al. Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation[J]. JOURNAL OF APPLIED PHYCOLOGY,2023:16.
APA	Jia, Shuao,Wu, Songcui,Liu, Xuehua,Gu, Wenhui,&Wang, Guangce.(2023).Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation.JOURNAL OF APPLIED PHYCOLOGY,16.
MLA	Jia, Shuao,et al."Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation".JOURNAL OF APPLIED PHYCOLOGY (2023):16.