Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle

doi:10.1016/j.snb.2023.133854

	Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle
	Zhou, Yanan 1,2,3,4; Zeng, Yan1,2,3 ; Wang, Peng1,2 ; Qi, Peng1,2,3 ; Sun, Yan 1,2,3; Zhang, Dun 1,2,3
	2023-09-01
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	390 页码:8
通讯作者	Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zengyan@qdio.ac.cn)
摘要	Here a colorimetric sensing platform for sulfate-reducing bacterial DNA analysis was described by using a catalytic hemin/G-quadruplex loaded rigid DNA triangle assembly. This two-dimensional rigid DNA triangle structure was assembled by target bacterial DNA and three hairpin beacon probes. As three tips of hairpin probes carry specific recognition sites for G-quadruplex formation, target bacterial DNA trigger the opening of hairpin probes, not only forming a steady rigid DNA triangle but also activating the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme. The resultant hemin/G-quadruplex loaded rigid DNA triangle assembly catalyzes a colorimetric reaction with the signal intensity correlated to target concentrations, achieving target bacterial DNA detection with a detection limit as low as 23.99 fM. We further analyzed actual bacteria with this system, by acquiring bacterial DNA including bacteria-specific sequences with polymerase chain reaction (PCR), with the detection limit down to 1 cfu/mL. The system shows good selectivity for the target and resistance to interference, highly stable performance for serum samples application, showing great potential for application in the fields of genetic analysis, environmental monitoring, and medical testing.
关键词	Rigid DNA triangle Hemin G-quadruplex Sulfate -reducing bacteria
DOI	10.1016/j.snb.2023.133854
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:001010022200001
出版者	ELSEVIER SCIENCE SA
WOS关键词	LABEL-FREE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182309
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zeng, Yan; Zhang, Dun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Yanan,Zeng, Yan,Wang, Peng,et al. Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,390:8.
APA	Zhou, Yanan,Zeng, Yan,Wang, Peng,Qi, Peng,Sun, Yan,&Zhang, Dun.(2023).Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle.SENSORS AND ACTUATORS B-CHEMICAL,390,8.
MLA	Zhou, Yanan,et al."Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle".SENSORS AND ACTUATORS B-CHEMICAL 390(2023):8.