Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)

doi:10.3389/fmars.2023.1183744

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)
	Wen, Jianqing1,2 ; Gong, Jinyu 2,3; Li, Pengwei 1; Deng, Penghui 1,2; Sun, Mengsi 4; Wu, Yujie 4; Tian, Chenxi 2,3; Wang, Hao 1; Bi, Yunchen 1,2,5
	2023-05-24
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	10 页码:13
通讯作者	Wang, Hao(wanghao@qdio.ac.cn) ; Bi, Yunchen(yunchenbi@qdio.ac.cn)
摘要	Elasmobranchs are crucial for comparative studies of evolution, as they belong to the most ancient vertebrate lineages that survived numerous extinction events and persist until today. The immunoglobulin new antigen receptor (IgNAR) found in sharks and heavy-chain-only antibody (HCAb) found in camelidae are products of convergent evolution. Although it was previously believed that IgNAR emerged 220 million years ago, before the divergence of sharks and skates, there is limited evidence to support this. In this study, we provide data supporting the existence of IgNAR in the ocellate spot skate (Okamejei kenojei) mononuclear cell transcriptome and peripheral blood serum. Additionally, we characterize the germline gene configuration of the ocellate spot skate IgNAR V domain. The ocellate spot skate IgNAR structure prediction and VNAR crystal structure exhibit high similarity to their shark counterparts. These data strongly suggest that IgNAR in both sharks and skates share a common ancestor. Sequencing of the ocellate spot skate VNAR repertoire provided crucial data for further understanding of the IgNAR generation. Notably, we discovered that approximately 99% of the ocellate spot skate VNARs belonged to type IV. This represents an exceptionally high proportion of type IV within the VNAR repertoire, which has not been documented in previously studied elasmobranchs. This unique characteristic of the ocellate spot skate VNAR adds essential structural diversity to the naive VNAR library from elasmobranchs and could potentially benefit the development of pharmaceutical drugs.
关键词	IgNAR VNAR skate VNAR structure antibody diversity
DOI	10.3389/fmars.2023.1183744
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876168] ; Taishan Young Scholar Program of Shandong Province[tsqn201812108] ; Hefei Science Center, CAS[2020HSC-UE018]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:001005205000001
出版者	FRONTIERS MEDIA SA
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; VARIABLE DOMAIN ANTIBODIES ; ANTIGEN RECEPTOR IGNAR ; LIGHT-CHAIN GENES ; SHARK IMMUNOGLOBULIN ; STRUCTURAL-ANALYSIS ; PROTEIN ; REARRANGEMENT ; DIVERSITY ; EXCLUSION
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182277
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Wang, Hao; Bi, Yunchen
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing, Peoples R China 4.Shenzhen Bay Lab, Dept Core Facil, Biochem Core, Shenzhen, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wen, Jianqing,Gong, Jinyu,Li, Pengwei,et al. Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)[J]. FRONTIERS IN MARINE SCIENCE,2023,10:13.
APA	Wen, Jianqing.,Gong, Jinyu.,Li, Pengwei.,Deng, Penghui.,Sun, Mengsi.,...&Bi, Yunchen.(2023).Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei).FRONTIERS IN MARINE SCIENCE,10,13.
MLA	Wen, Jianqing,et al."Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)".FRONTIERS IN MARINE SCIENCE 10(2023):13.