Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies

doi:10.1016/j.theriogenology.2023.05.003

IOCAS-IR > 实验海洋生物学重点实验室

	Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies
	Wang, Xueying1,2 ; Liu, Qinghua 1,2; Zhou, Li 1,2,3; Song, Zongcheng 4; Li, Jun 1,2
	2023-08-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	206 页码:189-196
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	Ovarian fluid is essential for successful fertilization by maintaining the viability, motility, and velocity of sperm. The organic compounds and inorganic ions in ovarian fluid significantly influence spermatozoa's motility, velocity, and longevity. However, the effect of ovarian fluid on sperm performance is limited in teleost fish. In this study, the effect of ovarian fluid on sperm performance and its components in external fertilization species (Scophthalmus maximus, turbot) and internal fertilization species (Sebastes schlegelii, black rockfish) was investigated using computer-assisted sperm analysis, high-performance liquid chromatography, and metabolome analysis. The ovarian fluid had a distinct and species-specific effect on both species. In the black rockfish, the ovarian fluid from turbot significantly increased sperm motility (74.07% +/- 4.09%), as well as VCL (45 +/- 1.67 mm/s), VAP (40.17 +/- 1.6 mm/s), and VSL (36.67 +/- 1.86 mm/s), and longevity (352 +/- 11.31 min) (P < 0.05). In the turbot, only the longevity (71.33 +/- 5.69 min) and fertilization rate (65.27% +/- 11.59%) showed significantly improvement (P < 0.05). The ovarian fluid was rich in organic compounds, suggesting enrichment in the glycolysis/gluconeogenesis pathways. The results suggest that glycometabolism plays a crucial role in improving sperm performance in teleost with internal fertilization. Thus, incorporating ovarian fluid into the sperm activation medium can enhance artificial fertilization in fish breeding.(c) 2023 Elsevier Inc. All rights reserved.
关键词	Turbot Black rockfish Sperm activation medium
DOI	10.1016/j.theriogenology.2023.05.003
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31802278] ; National Key R &D Program of China[2018YFD0901204] ; National Key R &D Program of China[2018YFD0901205] ; China Agriculture Research System[CARS-47] ; Major science and technology for scientific and technological innovation projects (Shandong)[2019JZZY020710]
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:001008080600001
出版者	ELSEVIER SCIENCE INC
WOS关键词	TURBOT SCOPHTHALMUS-MAXIMUS ; ONCORHYNCHUS-MYKISS WALBAUM ; RAINBOW-TROUT ; BIOCHEMICAL-COMPOSITION ; MOTILITY ; ACTIVATION ; GLYCOLYSIS ; QUALITY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182265
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xueying,Liu, Qinghua,Zhou, Li,et al. Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies[J]. THERIOGENOLOGY,2023,206:189-196.
APA	Wang, Xueying,Liu, Qinghua,Zhou, Li,Song, Zongcheng,&Li, Jun.(2023).Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies.THERIOGENOLOGY,206,189-196.
MLA	Wang, Xueying,et al."Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies".THERIOGENOLOGY 206(2023):189-196.