Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis

IOCAS-IR > 实验海洋生物学重点实验室

	Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis
	Ma, Qingjun; Akhter, Yusuf; Wilmanns, Matthias; Ehebauer, Matthias T.; Ma, Qingjun; Akhter, Yusuf; Wilmanns, Matthias; Ehebauer, Matthias T.
	2014-07
发表期刊	PROTEIN SCIENCE
卷号	23 期号:7 页码:932-939
摘要	; Protein biotinylation, a rare form of post-translational modification, is found in enzymes required for lipid biosynthesis. In mycobacteria, this process is essential for the formation of their complex and distinct cell wall and has become a focal point of drug discovery approaches. The enzyme responsible for this process, biotin protein ligase, substantially varies in different species in terms of overall structural organization, regulation of function and substrate specificity. To advance the understanding of the molecular mechanism of biotinylation in Mycobacterium tuberculosis we have biochemically and structurally characterized the corresponding enzyme. We report the high-resolution crystal structures of the apo-form and reaction intermediate biotinyl-5'-AMP-bound form of M. tuberculosis biotin protein ligase. Binding of the reaction intermediate leads to clear disorder-to-order transitions. We show that a conserved lysine, Lys138, in the active site is essential for biotinylation.
关键词	Mycobacterium Tuberculosis Bpl Bira Biotinylation Crystal Structure Acetyl-coa Carboxylase Escherichia-coli Holoenzyme Synthetase Carrier Protein Bira Gene Specificity Biotinylation Purification Activation Complexes
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/18197
专题	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Ma, Qingjun,Akhter, Yusuf,Wilmanns, Matthias,et al. Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis[J]. PROTEIN SCIENCE,2014,23(7):932-939.
APA	Ma, Qingjun.,Akhter, Yusuf.,Wilmanns, Matthias.,Ehebauer, Matthias T..,Ma, Qingjun.,...&Ehebauer, Matthias T..(2014).Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis.PROTEIN SCIENCE,23(7),932-939.
MLA	Ma, Qingjun,et al."Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis".PROTEIN SCIENCE 23.7(2014):932-939.

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Active site conforma（550KB）	期刊论文	出版稿	限制开放	ODC BY	浏览