Developmental regulation of the male urogenital papilla in the male marine teleost black rockfish, Sebastes schlegelii (Hilgendorf, 1880)

doi:10.1093/biolre/ioad086

IOCAS-IR > 实验海洋生物学重点实验室

	Developmental regulation of the male urogenital papilla in the male marine teleost black rockfish, Sebastes schlegelii (Hilgendorf, 1880)
	Wang, Tao 1,2,3,4; Wang, Xueying1,2,4,7,8 ; Zhao, Ning 1,2,4,5; Liu, Qinghua 1,2,4; Song, Zongcheng 6; Li, Jun 1,2,4,7,8
	2023-08-08
发表期刊	BIOLOGY OF REPRODUCTION
ISSN	0006-3363
页码	13
通讯作者	Wang, Xueying(xueyingwang@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	The male external genitalia of the black rockfish (Sebastes schlegelii Hilgendorf, 1880) is a fleshy protrusion known as the urogenital papilla (UGP), which functions to deliver sperm into the female reproductive tract for internal fertilization. It is not known which genes regulate the development of the UGP. The aim of this study was to identify key genes that regulate the development of the UGP in black rockfish and to determine the distribution of androgen receptor gene (ar) in the UGP. A total of 26 adult males and 560 juvenile fish were used in the experiment, in which we divided all normally developing juveniles into normal development and androgen groups. We added methyltestosterone solution (100 mu g/l) to the androgen group-treated fish tank, soaked for 2 h per day for 38 days, and sampled 5 similar to 10 samples each time every 5 days during the culture process. Gene expression changes related to UGP were analyzed with tissue specificity between control and androgen groups during sex differentiation, adult male maturation, and the copulation stage (September to December) using real-time quantitative polymerase chain reaction. The expression of ar was also localized by two-color in situ hybridization in the UGP region of juvenile fish. Androgen treatment enhanced ar expression levels and the ar signal was stronger in the UGP region of both adult breeding fish and androgen-treated juvenile fish. This study provides insights into the regulation of the external genitalia of black rockfish and presents vital information for the artificial breeding of viviparous fish.
关键词	androgen external genitalia viviparous ara/arb/mafb/shha
DOI	10.1093/biolre/ioad086
收录类别	SCI
语种	英语
WOS研究方向	Reproductive Biology
WOS类目	Reproductive Biology
WOS记录号	WOS:001061381600001
出版者	OXFORD UNIV PRESS INC
WOS关键词	SEXUALLY DIMORPHIC EXPRESSION ; MESSENGER-RNA LEVELS ; ANDROGEN RECEPTOR ; PHYLOGENETIC ANALYSIS ; CDNA CLONING ; JUVENILE ; FISH ; DIFFERENTIATION ; MASCULINIZATION ; MECHANISM
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181839
专题	实验海洋生物学重点实验室
通讯作者	Wang, Xueying; Li, Jun
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China 7.Chinese Acad Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 8.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Tao,Wang, Xueying,Zhao, Ning,et al. Developmental regulation of the male urogenital papilla in the male marine teleost black rockfish, Sebastes schlegelii (Hilgendorf, 1880)[J]. BIOLOGY OF REPRODUCTION,2023:13.
APA	Wang, Tao,Wang, Xueying,Zhao, Ning,Liu, Qinghua,Song, Zongcheng,&Li, Jun.(2023).Developmental regulation of the male urogenital papilla in the male marine teleost black rockfish, Sebastes schlegelii (Hilgendorf, 1880).BIOLOGY OF REPRODUCTION,13.
MLA	Wang, Tao,et al."Developmental regulation of the male urogenital papilla in the male marine teleost black rockfish, Sebastes schlegelii (Hilgendorf, 1880)".BIOLOGY OF REPRODUCTION (2023):13.