Comparative analysis of the mitochondrial genomes of the family Mactridae (Mollusca: Venerida) and their phylogenetic implications

doi:10.1016/j.ijbiomac.2023.126081

	Comparative analysis of the mitochondrial genomes of the family Mactridae (Mollusca: Venerida) and their phylogenetic implications
	Ma, Peizhen1 ; Liu, Yumeng1 ; Wang, Jiahui1,2 ; Chen, Ya 1,3; Zhang, Zhen 1,3; Zhang, Tao4 ; Wang, Haiyan 1
	2023-09-30
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	249 页码:9
通讯作者	Wang, Haiyan(haiyanwang@qdio.ac.cn)
摘要	Taxonomy and phylogenetic relationships within the family Mactridae have remained debatable because of the plasticity of morphological characteristics and the lack of accurate molecular data, thereby resulting in abundant synonyms and taxa rearrangements. Mitochondrial genomes (mitogenomes) have been widely used as powerful tools to reconstruct phylogenies of various groups of mollusks; however, they have not been used for studying the phylogeny of mactrids specifically. In the present study, mitogenomes of seven Mactridae species, namely Mactra chinensis, Mactra cygnus, Mactra quadrangularis, Mactra cumingii, Mactrinula dolabrata, Raeta pulchella, and Raeta sp., were sequenced by Illumina high-throughput sequencing, and a comparative mitochondrial genomic analysis was conducted. The newly sequenced mitogenomes were double-stranded circular molecules, with all functional genes encoded on the heavy strand. All the new mactrid mitogenomes had two rRNA genes (12S and 16S), 13 protein-coding genes (PCGs) (atp6, cox1, cox2, cox3, cytb, nad1, nad2, nad3, nad4, nad4l, nad5, nad6, and atp8), and 22 tRNAs. The mitogenomes showed considerable variation in AT content, GC skew, and AT skew. The results of the phylogenetic analysis confirmed monophyly of the family Mactridae and suggested that genera Mactrinula, Spisula, Rangia, and Mulinia should not be placed under subfamily Mactrinae. Our results supported that potential cryptic species existed in Mactra antiquata. We also proposed subfamily Kymatoxinae should belong to the family Mactridae rather than Anatinellidae and Mactra alta in China should be Mactra cygnus. Additionally, conservation in functional gene arrangement was found in genera Mactra, Raeta, and Lutraria. But gene orders in S. sachalinensis and S. solida were quite different, questioning their congeneric relationship. Our results further suggested that the taxonomy within the family Mactridae requires an integrative revision.
关键词	Mactridae Mitochondrial genome Gene arrangement Phylogeny Mollusk
DOI	10.1016/j.ijbiomac.2023.126081
收录类别	SCI
语种	英语
资助项目	Science & Technology Fundamental Resources Investigation Program[2022FY100300] ; applied research projects for postdoctoral researchers in Qingdao, Liaoning Provincial projects to promote marine economic development ; Liaoning Provincial Central Government Guides Local Science and Technology Development Special Project ; earmarked fund for CARS[CARS-49] ; Guangxi Key Research and Development Program[2021AB34014]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001064697700001
出版者	ELSEVIER
WOS关键词	GENE ORDER ; DNA-SEQUENCE ; EVOLUTION ; BIVALVIA ; INFERENCE ; DIFFERENTIATION ; REARRANGEMENTS ; DIVERSITY ; ALGORITHM ; PROTEIN
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181824
专题	海洋生物分类与系统演化实验室海洋生态与环境科学重点实验室
通讯作者	Wang, Haiyan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ma, Peizhen,Liu, Yumeng,Wang, Jiahui,et al. Comparative analysis of the mitochondrial genomes of the family Mactridae (Mollusca: Venerida) and their phylogenetic implications[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,249:9.
APA	Ma, Peizhen.,Liu, Yumeng.,Wang, Jiahui.,Chen, Ya.,Zhang, Zhen.,...&Wang, Haiyan.(2023).Comparative analysis of the mitochondrial genomes of the family Mactridae (Mollusca: Venerida) and their phylogenetic implications.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,249,9.
MLA	Ma, Peizhen,et al."Comparative analysis of the mitochondrial genomes of the family Mactridae (Mollusca: Venerida) and their phylogenetic implications".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 249(2023):9.