Expression patterns and antifungal function study of KaSPI in <i>Mythimna separata</i>

doi:10.1017/S000748532300041

IOCAS-IR > 实验海洋生物学重点实验室

	Expression patterns and antifungal function study of KaSPI in Mythimna separata
	Chen, Ya-Ru 1,2; Yang, Hong-Jia 1; Cha, Jin-Myong 1,3; Zhang, Xin-Xin 1; Fan, Dong 1
	2023-09-21
发表期刊	BULLETIN OF ENTOMOLOGICAL RESEARCH
ISSN	0007-4853
页码	11
通讯作者	Zhang, Xin-Xin(xinxinz@neau.edu.cn) ; Fan, Dong(dnfd@163.com)
摘要	Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of Mythimna separata, the cDNA sequence of MsKaSPI with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that MsKaSPI was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of M. separata. After 24 h infection by Beauveria bassiana, the expression level of MsKaSPI and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with MsKaSPI for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of M. separata were prolonged, the weights were reduced and the number of eggs per female decreased by 181. Beauveria bassiana infection also increased the mortality of MsKaSPI-silenced M. separata by 18.96%. These prove MsKaSPI can not only result in slow growth and low fecundity of M. separata by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.
关键词	Beauveria bassiana enzyme activity Mythimna separata RNA interference serine protease inhibitor
DOI	10.1017/S000748532300041
收录类别	SCI
语种	英语
资助项目	This work was supported financially by the National Key Ramp;amp;D Program of China (2018YFD0201000) and Special Fund for the Construction of Modern Agricultural Industrial Technology System (CARS-04).[2018YFD0201000] ; National Key Ramp;amp;D Program of China[CARS-04] ; Special Fund for the Construction of Modern Agricultural Industrial Technology System
WOS研究方向	Entomology
WOS类目	Entomology
WOS记录号	WOS:001068923700001
出版者	CAMBRIDGE UNIV PRESS
WOS关键词	SERINE-PROTEASE INHIBITOR ; IMMUNE-RESPONSE ; ECDYSONE ; GENE ; HORMONE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181787
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xin-Xin; Fan, Dong
作者单位	1.Northeast Agr Univ, Coll Plant Protect, Harbin 150030, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Kyeungsang Sariwon Agr Univ, Pyongyang 95003, North Korea
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Ya-Ru,Yang, Hong-Jia,Cha, Jin-Myong,et al. Expression patterns and antifungal function study of KaSPI in Mythimna separata[J]. BULLETIN OF ENTOMOLOGICAL RESEARCH,2023:11.
APA	Chen, Ya-Ru,Yang, Hong-Jia,Cha, Jin-Myong,Zhang, Xin-Xin,&Fan, Dong.(2023).Expression patterns and antifungal function study of KaSPI in Mythimna separata.BULLETIN OF ENTOMOLOGICAL RESEARCH,11.
MLA	Chen, Ya-Ru,et al."Expression patterns and antifungal function study of KaSPI in Mythimna separata".BULLETIN OF ENTOMOLOGICAL RESEARCH (2023):11.