Isolation and characterization of glutathione S-transferase genes and their transcripts in <i>Saccharina japonica</i> (Laminariales, Phaeophyceae) during development and under abiotic stress

doi:10.1186/s12870-023-04430-5

IOCAS-IR > 实验海洋生物学重点实验室

	*Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica* (Laminariales, Phaeophyceae) during development and under abiotic stress**
	Lu, Chang1,2 ; Zhang, Pengyan3,4 ; Li, Shuang1,3,5 ; Cheng, Mengzhen1,3,5 ; Duan, Delin 1
	2023-09-18
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	23 期号:1 页码:19
通讯作者	Duan, Delin(dlduan@qdio.ac.cn)
摘要	Background Glutathione S-transferase (GST) is a crucial enzyme for metabolism, detoxification, and stress resistance in organisms. Many GSTs have been identified in seaweeds, but the isolation and functional analysis of GSTs in Saccharina japonica have not been completed. Result In this study, a total of 32 SjGST genes, localized on 10 scaffolds and 6 contigs, were identified and categorized into three groups. Most of these SjGSTs were presumed to be distributed in the cytoplasm. Tandem duplication had a significant influence on the expansion of the SjGST gene family. Functional analysis of cis-acting elements in the promoter regions demonstrated that SjGSTs enhance the stress resistance of the kelp. Quantitative real-time PCR tests confirmed that SjGSTs positively influence S. japonica sporophytes under stress from low salinity, drought, and high temperature. Recombinant yeast tests further affirmed the role of SjGSTs in stress resistance; SjGSTs improved the growth rate of recombinant yeast under 1.5 M NaCl or 8 mM H2O2. Analysis of biochemical parameters indicated that the optimum temperatures for SjGST20 and SjGST22 were 20 degrees C, and the optimum pH values were 7.0 and 8.0 for SjGST20 and SjGST22, respectively. The K-m values for the substrate 1-chloro-2,4-dinitrobenzene (CDNB) were 2.706 mM and 0.674 mM and were 6.146 mM and 3.559 mM for the substrate glutathione (GSH) for SjGST20 and SjGST22, respectively. Conclusion SjGSTs are important stress resistant genes in S. japonica. This research results will enhance our understanding the function of GSTs in brown seaweeds, and explained its functional roles in stress resistance in marine environments.
关键词	Saccharina japonica Glutathione S-transferase Transcriptome Gene expression Abiotic stresses Enzyme activity assay
DOI	10.1186/s12870-023-04430-5
收录类别	SCI
语种	英语
资助项目	Joint Research Project between China and Japan[2017YFE0130900] ; Qingdao International Innovation Cooperation Project for Science and Technology[22-3-6-ghgg-1-Hz]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001068658100001
出版者	BMC
WOS关键词	GENOME-WIDE IDENTIFICATION ; GST-FAMILY ; EXPRESSION ; TOLERANCE ; OVEREXPRESSION ; DATABASE ; MEMBERS ; SALT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181719
专题	实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 2.Yantai Univ, Coll Life Sci, Dept Biol Engn, Yantai 264005, Peoples R China 3.Natl Lab Marine Sci & Technol, Funct Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Div Mariculture Ecol & Technol, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lu, Chang,Zhang, Pengyan,Li, Shuang,et al. Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress[J]. BMC PLANT BIOLOGY,2023,23(1):19.
APA	Lu, Chang,Zhang, Pengyan,Li, Shuang,Cheng, Mengzhen,&Duan, Delin.(2023).Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress.BMC PLANT BIOLOGY,23(1),19.
MLA	Lu, Chang,et al."Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress".BMC PLANT BIOLOGY 23.1(2023):19.