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Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress
Lu, Chang1,2; Zhang, Pengyan3,4; Li, Shuang1,3,5; Cheng, Mengzhen1,3,5; Duan, Delin1
2023-09-18
Source PublicationBMC PLANT BIOLOGY
ISSN1471-2229
Volume23Issue:1Pages:19
Corresponding AuthorDuan, Delin(dlduan@qdio.ac.cn)
AbstractBackground Glutathione S-transferase (GST) is a crucial enzyme for metabolism, detoxification, and stress resistance in organisms. Many GSTs have been identified in seaweeds, but the isolation and functional analysis of GSTs in Saccharina japonica have not been completed. Result In this study, a total of 32 SjGST genes, localized on 10 scaffolds and 6 contigs, were identified and categorized into three groups. Most of these SjGSTs were presumed to be distributed in the cytoplasm. Tandem duplication had a significant influence on the expansion of the SjGST gene family. Functional analysis of cis-acting elements in the promoter regions demonstrated that SjGSTs enhance the stress resistance of the kelp. Quantitative real-time PCR tests confirmed that SjGSTs positively influence S. japonica sporophytes under stress from low salinity, drought, and high temperature. Recombinant yeast tests further affirmed the role of SjGSTs in stress resistance; SjGSTs improved the growth rate of recombinant yeast under 1.5 M NaCl or 8 mM H2O2. Analysis of biochemical parameters indicated that the optimum temperatures for SjGST20 and SjGST22 were 20 degrees C, and the optimum pH values were 7.0 and 8.0 for SjGST20 and SjGST22, respectively. The K-m values for the substrate 1-chloro-2,4-dinitrobenzene (CDNB) were 2.706 mM and 0.674 mM and were 6.146 mM and 3.559 mM for the substrate glutathione (GSH) for SjGST20 and SjGST22, respectively. Conclusion SjGSTs are important stress resistant genes in S. japonica. This research results will enhance our understanding the function of GSTs in brown seaweeds, and explained its functional roles in stress resistance in marine environments.
KeywordSaccharina japonica Glutathione S-transferase Transcriptome Gene expression Abiotic stresses Enzyme activity assay
DOI10.1186/s12870-023-04430-5
Indexed BySCI
Language英语
Funding ProjectJoint Research Project between China and Japan[2017YFE0130900] ; Qingdao International Innovation Cooperation Project for Science and Technology[22-3-6-ghgg-1-Hz]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:001068658100001
PublisherBMC
WOS KeywordGENOME-WIDE IDENTIFICATION ; GST-FAMILY ; EXPRESSION ; TOLERANCE ; OVEREXPRESSION ; DATABASE ; MEMBERS ; SALT
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/181719
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan, Delin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China
2.Yantai Univ, Coll Life Sci, Dept Biol Engn, Yantai 264005, Peoples R China
3.Natl Lab Marine Sci & Technol, Funct Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Div Mariculture Ecol & Technol, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lu, Chang,Zhang, Pengyan,Li, Shuang,et al. Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress[J]. BMC PLANT BIOLOGY,2023,23(1):19.
APA Lu, Chang,Zhang, Pengyan,Li, Shuang,Cheng, Mengzhen,&Duan, Delin.(2023).Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress.BMC PLANT BIOLOGY,23(1),19.
MLA Lu, Chang,et al."Isolation and characterization of glutathione S-transferase genes and their transcripts in Saccharina japonica (Laminariales, Phaeophyceae) during development and under abiotic stress".BMC PLANT BIOLOGY 23.1(2023):19.
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