IOCAS-IR  > 海洋环境腐蚀与生物污损重点实验室
Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection
Zeng, Yan1,2,3; Qi, Peng1,2,3; Chen, Jiawei1,2,3; Wang, Peng1,2,3; Zhang, Dun1,2,3
2023-12-01
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
Volume396Pages:7
Corresponding AuthorQi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractQuantity analysis of bacterial 16 S rDNA gene is still a challenge. Here we successfully achieved bacterial 16 S rDNA gene SERS analysis with target aided self-assembly of DNA hyperbranched nanostructures. Catalytic hairpin assembly (CHA) was first introduced for self-assembly of branched nanostructures, including target bacterial DNA served as trigger probe and three hairpin probes for further target activated cross opening. Rolling cycle amplification (RCA) was then introduced by using a circular DNA as template and single stranded branches of the formed branched nanostructures as primer, forming a concatemer containing multiple copies of tandem repeating segments with same sequences complementary to the circular templates. After that, thousands of Raman-Au nanoprobe would hybridize this long single stranded DNAs for DNA-AuNPs nanoaggretates assembly. By using SERS signal collection, target bacterial DNAs would be successfully analyzed with ultra-sensitivity as low as 15.0 fM. Furthermore, selectivity test for targets with mismatched DNA sequences and other bacterial 16 S rDNA genes show good results. This sensing method was extended to quantify target genes in human plasma samples, showing its excellent applicability in complex samples.
KeywordDNA hyperbranched nanostructure SERS Target aided Bacterial 16 S rDNA genes
DOI10.1016/j.snb.2023.134423
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[42376208] ; National Natural Science Foundation of China[41922040]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:001075737400001
PublisherELSEVIER SCIENCE SA
WOS KeywordGOLD NANOPARTICLES ; AMPLIFICATION
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/181707
Collection海洋环境腐蚀与生物污损重点实验室
Corresponding AuthorQi, Peng; Zhang, Dun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zeng, Yan,Qi, Peng,Chen, Jiawei,et al. Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,396:7.
APA Zeng, Yan,Qi, Peng,Chen, Jiawei,Wang, Peng,&Zhang, Dun.(2023).Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection.SENSORS AND ACTUATORS B-CHEMICAL,396,7.
MLA Zeng, Yan,et al."Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection".SENSORS AND ACTUATORS B-CHEMICAL 396(2023):7.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S092540052301(4064KB)期刊论文出版稿限制开放CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zeng, Yan]'s Articles
[Qi, Peng]'s Articles
[Chen, Jiawei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zeng, Yan]'s Articles
[Qi, Peng]'s Articles
[Chen, Jiawei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zeng, Yan]'s Articles
[Qi, Peng]'s Articles
[Chen, Jiawei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0925400523011383-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.