Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection | |
Zeng, Yan1,2,3; Qi, Peng1,2,3; Chen, Jiawei1,2,3; Wang, Peng1,2,3; Zhang, Dun1,2,3 | |
2023-12-01 | |
发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL |
卷号 | 396页码:7 |
通讯作者 | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
摘要 | Quantity analysis of bacterial 16 S rDNA gene is still a challenge. Here we successfully achieved bacterial 16 S rDNA gene SERS analysis with target aided self-assembly of DNA hyperbranched nanostructures. Catalytic hairpin assembly (CHA) was first introduced for self-assembly of branched nanostructures, including target bacterial DNA served as trigger probe and three hairpin probes for further target activated cross opening. Rolling cycle amplification (RCA) was then introduced by using a circular DNA as template and single stranded branches of the formed branched nanostructures as primer, forming a concatemer containing multiple copies of tandem repeating segments with same sequences complementary to the circular templates. After that, thousands of Raman-Au nanoprobe would hybridize this long single stranded DNAs for DNA-AuNPs nanoaggretates assembly. By using SERS signal collection, target bacterial DNAs would be successfully analyzed with ultra-sensitivity as low as 15.0 fM. Furthermore, selectivity test for targets with mismatched DNA sequences and other bacterial 16 S rDNA genes show good results. This sensing method was extended to quantify target genes in human plasma samples, showing its excellent applicability in complex samples. |
关键词 | DNA hyperbranched nanostructure SERS Target aided Bacterial 16 S rDNA genes |
DOI | 10.1016/j.snb.2023.134423 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[42376208] ; National Natural Science Foundation of China[41922040] |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
WOS记录号 | WOS:001075737400001 |
出版者 | ELSEVIER SCIENCE SA |
WOS关键词 | GOLD NANOPARTICLES ; AMPLIFICATION |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/181707 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Qi, Peng; Zhang, Dun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Zeng, Yan,Qi, Peng,Chen, Jiawei,et al. Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,396:7. |
APA | Zeng, Yan,Qi, Peng,Chen, Jiawei,Wang, Peng,&Zhang, Dun.(2023).Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection.SENSORS AND ACTUATORS B-CHEMICAL,396,7. |
MLA | Zeng, Yan,et al."Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection".SENSORS AND ACTUATORS B-CHEMICAL 396(2023):7. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
1-s2.0-S092540052301(4064KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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