Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression

doi:10.1002/jsfa.12986

IOCAS-IR > 实验海洋生物学重点实验室

	Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression
	Song, Wen 3,4,5,6; Xing, Ronge1,2,3,4,5 ; Yang, Haoyue3,4 ; Liu, Song 3,4,5; Yu, Huahua3,4,5 ; Li, Pengcheng3,4,5
	2023-09-29
发表期刊	JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
ISSN	0022-5142
页码	15
通讯作者	Xing, Ronge(xingronge@qdio.ac.cn)
摘要	Background: Type 2 diabetes mellitus (T2DM) is a prevalent metabolic disease that poses significant health risks due to its numerous complications. However, the effects of eumelanin on oxidative stress, hyperglycemia and depression in diabetic mice have not been extensively studied. Results: Our study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline-neutral-flavor protease (ANF) displayed superior inhibitory activity against alpha-glucosidase and alpha-amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance and modulating total cholesterol and low-density lipoprotein levels, as well as antioxidant indices at a dosage of 0.04 g kg(-1). Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice. Conclusion: Overall, these findings underscore the significant therapeutic potential of ANF in the treatment of T2DM and its associated complications. By augmenting lipid and glucose metabolism, mitigating oxidative stress and alleviating depression, ANF emerges as a promising candidate for multifaceted intervention. (c) 2023 Society of Chemical Industry.
关键词	type 2 diabetes mellitus (T2DM) eumelanin hypoglycemic activity antioxidant activity antidepressant activity
DOI	10.1002/jsfa.12986
收录类别	SCI
语种	英语
资助项目	14th Five-Year Plan of Qingdao Pilot National Laboratory of Marine Science and Technology[2022QNLM03003-2] ; Shandong Key RD Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Fujian Science and Technology Planning Project-STS Program[2021 T3013]
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:001075206100001
出版者	WILEY
WOS关键词	ALPHA-GLUCOSIDASE ; MELANIN ; MODEL ; AMYLASE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181704
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Wen,Xing, Ronge,Yang, Haoyue,et al. Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,2023:15.
APA	Song, Wen,Xing, Ronge,Yang, Haoyue,Liu, Song,Yu, Huahua,&Li, Pengcheng.(2023).Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,15.
MLA	Song, Wen,et al."Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023):15.