IOCAS-IR  > 海洋生物分类与系统演化实验室
Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation
Wang, Lulu1; Sun, Yan2; Zhang, Ruihao1; Pan, Kehou1,3; Li, Yuhang4; Wang, Ruibing5; Zhang, Lin6; Zhou, Chengxu2; Li, Jian7; Li, Yun1; Zhu, Baohua1; Han, Jichang2
Corresponding AuthorHan, Jichang(
AbstractBackground The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type ( WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism.
KeywordDiatom frustule Silica Morphology remolding Genetic modification Hemostatic material
Indexed BySCI
Funding ProjectNot applicable.
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:001067101000001
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Document Type期刊论文
Corresponding AuthorHan, Jichang
Affiliation1.Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China
2.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315200, Peoples R China
3.Laoshan Lab, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Dept Marine Organism Taxon & Phylogeny, Inst Oceanol, Qingdao 266071, Peoples R China
5.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China
6.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Ningbo 315200, Peoples R China
7.Panzhihua Univ, Sch Biol & Chem Engn, Panzhihua 617000, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lulu,Sun, Yan,Zhang, Ruihao,et al. Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation[J]. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,2023,16(1):21.
APA Wang, Lulu.,Sun, Yan.,Zhang, Ruihao.,Pan, Kehou.,Li, Yuhang.,...&Han, Jichang.(2023).Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation.BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,16(1),21.
MLA Wang, Lulu,et al."Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation".BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 16.1(2023):21.
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