Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum

doi:10.1016/j.algal.2023.103270

IOCAS-IR > 实验海洋生物学重点实验室

	Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum
	Yanga, Wenting 1,2,4; Zhou, Lu 1,2,3; Zhuo, Jintao 1,2,3; Li, Xin1,2,3 ; Wu, Songcui1,2 ; Yu, Guihong ; Gao, Shan 1,2; Wang, Guangce 1,2
	2023-09-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	75 页码:7
通讯作者	Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Antimicrobial peptides (AMPs) are small-molecule polypeptides that occur widely in nature, have a broad antibacterial spectrum, and do not induce drug resistance in bacteria. Therefore, they are potential substitutes for antibiotics. However, there have been few reports of the production of the AMPs secreted by diatoms. Phaeodactylum tricornutum is a model diatom species for which protocols for its transformation are well established, and it can be directly used as fish and shrimp bait. In this study, we used P. tricornutum as a cell factory to produce secreted AMPs extracellularly. We also constructed an expression vector containing the secretory promoter and signal peptide of the Scygonadin gene, and successfully expressed AMPs in P. tricornutum, confirmed at the levels of DNA, RNA, and protein. Bacteriostatic experiments showed that the secreted AMPs exerted an obvious antibacterial effect on Kocuria sp. and Trelief5 alpha Chemical Competent Cells. This study demonstrates that P. tricornutum effectively expresses and secretes AMPs with obvious antibacterial effects.
关键词	Phaeodactylum tricornutum Secretory antimicrobial peptide Antibacterial
DOI	10.1016/j.algal.2023.103270
收录类别	SCI
语种	英语
资助项目	National Natural Science Founda- tion of China[41976097] ; National Key R & D Program of China[2018YFD0901500]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:001083333200001
出版者	ELSEVIER
WOS关键词	TRANSFORMATION ; MECHANISMS ; GENE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181623
专题	实验海洋生物学重点实验室
通讯作者	Gao, Shan; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yanga, Wenting,Zhou, Lu,Zhuo, Jintao,et al. Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,75:7.
APA	Yanga, Wenting.,Zhou, Lu.,Zhuo, Jintao.,Li, Xin.,Wu, Songcui.,...&Wang, Guangce.(2023).Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,75,7.
MLA	Yanga, Wenting,et al."Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 75(2023):7.