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Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments
Zhou, Cong1,2,3,4,5,6; Yang, Mei-jie1,2,3,4,5,6; Hu, Zhi1,2,3,4,5,6; Zou, Yan7; Shi, Pu1,2,3,4,5,6; Li, Yong-ren8; Guo, Yong-jun8; Song, Hao1,2,3,4,6,9; Zhang, Tao1,2,3,4,6,9
2023-11-01
Source PublicationMARINE ENVIRONMENTAL RESEARCH
ISSN0141-1136
Volume192Pages:11
Corresponding AuthorSong, Hao(haosong@qdio.ac.cn) ; Zhang, Tao(tzhang@qdio.ac.cn)
AbstractMarine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for osmoregulation. Euryhaline bivalves are capable of withstanding hyposaline and hypersaline environments through regulation of physiology, metabolism, and gene expression. However, current understanding of the molecular mechanisms underlying osmoregulation and salinity adaptation in euryhaline bivalves remains largely limited. In this study, RNA-seq, WGCNA and flow cytometric analysis were performed to investigate the physiological responses of hard clams (Mercenaria mercenaria) to acute short-term hyposalinity (AL) and hypersalinity (AH), and chronic long-term hyposalinity (CL) and hypersalinity (CH) stress. We found that amino acids biosynthesis was significantly inhibited and aminoacyl-tRNA biosynthesis was augmented to decrease intracellular osmolarity during hypo saline exposure. Under CH, numerous autophagy-related genes (ATGs) were highly expressed, and the autophagy activity of gill cells were significantly up-regulated. A significant decrease in total FAAs content was observed in gills after NH4Cl treatment, indicating that autophagy was crucial for osmoregulation in hard clams during prolonged exposure to hypersaline environments. To prevent premature or unnecessary apoptosis, the expression of cathepsin L was inhibited under AL and AH, and inhibitors of apoptosis was augmented under CL and CH. Additionally, neuroendocrine regulation was involved in salinity adaption in hard clams. This study provides novel insights into the physiological responses of euryhaline marine bivalves to hyposaline and hypersaline environments.
KeywordHyposalinity Hypersalinity Transcriptome Autophagy Free amino acids
DOI10.1016/j.marenvres.2023.106198
Indexed BySCI
Language英语
Funding ProjectTianjin science and technology commission project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; Key Research and Development Program of Guangxi Province[LMEES-CTSP-2018-1] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021QNRC001] ; Young Elite Scientists Sponsorship Program by CAST[CARS-49] ; Youth Innovation Promotion Association CAS ; earmarked fund for CARS ; [2021AB34014]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology ; Toxicology
WOS IDWOS:001085440300001
PublisherELSEVIER SCI LTD
WOS KeywordOYSTER CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; MYTILUS-GALLOPROVINCIALIS ; ENDOPLASMIC-RETICULUM ; MACOMA-BALTHICA ; STRESS ; EXPRESSION ; SALINITY ; ADAPTATION ; MECHANISMS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/181620
Collection海洋生态与环境科学重点实验室
Corresponding AuthorSong, Hao; Zhang, Tao
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
7.Marine Sci Res Inst Shandong Prov, Qingdao 266100, Peoples R China
8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China
9.7 Nanhai Rd, Qingdao, Shandong, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Cong,Yang, Mei-jie,Hu, Zhi,et al. Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments[J]. MARINE ENVIRONMENTAL RESEARCH,2023,192:11.
APA Zhou, Cong.,Yang, Mei-jie.,Hu, Zhi.,Zou, Yan.,Shi, Pu.,...&Zhang, Tao.(2023).Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments.MARINE ENVIRONMENTAL RESEARCH,192,11.
MLA Zhou, Cong,et al."Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments".MARINE ENVIRONMENTAL RESEARCH 192(2023):11.
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