Autophagy contributes to increase the content of intracellular free amino acids in hard clam<i> (Mercenaria</i><i> mercenaria)</i> during prolonged exposure to hypersaline environments

doi:10.1016/j.marenvres.2023.106198

	Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments
	Zhou, Cong1,2,3,4,5,6 ; Yang, Mei-jie 1,2,3,4,5,6; Hu, Zhi 1,2,3,4,5,6; Zou, Yan 7; Shi, Pu1,2,3,4,5,6 ; Li, Yong-ren 8; Guo, Yong-jun 8; Song, Hao 1,2,3,4,6,9; Zhang, Tao1,2,3,4,6,9
	2023-11-01
发表期刊	MARINE ENVIRONMENTAL RESEARCH
ISSN	0141-1136
卷号	192 页码:11
通讯作者	Song, Hao(haosong@qdio.ac.cn) ; Zhang, Tao(tzhang@qdio.ac.cn)
摘要	Marine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for osmoregulation. Euryhaline bivalves are capable of withstanding hyposaline and hypersaline environments through regulation of physiology, metabolism, and gene expression. However, current understanding of the molecular mechanisms underlying osmoregulation and salinity adaptation in euryhaline bivalves remains largely limited. In this study, RNA-seq, WGCNA and flow cytometric analysis were performed to investigate the physiological responses of hard clams (Mercenaria mercenaria) to acute short-term hyposalinity (AL) and hypersalinity (AH), and chronic long-term hyposalinity (CL) and hypersalinity (CH) stress. We found that amino acids biosynthesis was significantly inhibited and aminoacyl-tRNA biosynthesis was augmented to decrease intracellular osmolarity during hypo saline exposure. Under CH, numerous autophagy-related genes (ATGs) were highly expressed, and the autophagy activity of gill cells were significantly up-regulated. A significant decrease in total FAAs content was observed in gills after NH4Cl treatment, indicating that autophagy was crucial for osmoregulation in hard clams during prolonged exposure to hypersaline environments. To prevent premature or unnecessary apoptosis, the expression of cathepsin L was inhibited under AL and AH, and inhibitors of apoptosis was augmented under CL and CH. Additionally, neuroendocrine regulation was involved in salinity adaption in hard clams. This study provides novel insights into the physiological responses of euryhaline marine bivalves to hyposaline and hypersaline environments.
关键词	Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids
DOI	10.1016/j.marenvres.2023.106198
收录类别	SCI
语种	英语
资助项目	Tianjin science and technology commission project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; Key Research and Development Program of Guangxi Province[LMEES-CTSP-2018-1] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021QNRC001] ; Young Elite Scientists Sponsorship Program by CAST[CARS-49] ; Youth Innovation Promotion Association CAS ; earmarked fund for CARS ; [2021AB34014]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:001085440300001
出版者	ELSEVIER SCI LTD
WOS关键词	OYSTER CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; MYTILUS-GALLOPROVINCIALIS ; ENDOPLASMIC-RETICULUM ; MACOMA-BALTHICA ; STRESS ; EXPRESSION ; SALINITY ; ADAPTATION ; MECHANISMS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181620
专题	海洋生态与环境科学重点实验室
通讯作者	Song, Hao; Zhang, Tao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Marine Sci Res Inst Shandong Prov, Qingdao 266100, Peoples R China 8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China 9.7 Nanhai Rd, Qingdao, Shandong, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Cong,Yang, Mei-jie,Hu, Zhi,et al. Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments[J]. MARINE ENVIRONMENTAL RESEARCH,2023,192:11.
APA	Zhou, Cong.,Yang, Mei-jie.,Hu, Zhi.,Zou, Yan.,Shi, Pu.,...&Zhang, Tao.(2023).Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments.MARINE ENVIRONMENTAL RESEARCH,192,11.
MLA	Zhou, Cong,et al."Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments".MARINE ENVIRONMENTAL RESEARCH 192(2023):11.

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