Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus

doi:10.1016/j.ecoenv.2022.114419

	Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus
	Huo, Da 1,2,3,4,6; Su, Fang 1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7 ; Sun, Lina 1,2,3,4,5,6,8
	2023
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	249 页码:10
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
摘要	Exosomes are small extracellular vesicles that contain nucleic acids such as microRNAs and may participate in important biological processes. We made the initial report of exosomes from sea cucumber Apostichopus japo-nicus, that were classically cup-shaped and had an average size of 74.65 nm, and identified specific exosome biomarkers (HSP70, TSG101, and CD9). We explored changes in the global expression of microRNAs in exosomes from the commercially important A. japonicus under normal conditions and heat-stressed conditions for 3 and 7 d. We found that heat stress increased exosome production and modified the expression profiles of the micro-RNAs that they contained. Novel_mir31, novel_mir132, novel_mir26, miR-92_1, and novel_mir27 were commonly found to be differentially expressed in three comparison groups, indicating their importance in the heat stress response. The microRNA expression levels were validated by qPCR. Function analysis of the target genes of these microRNAs indicated they were involved mainly in replication and repair in the initial response of A. japonicus to heat stress exposure. Conversely, during acclimation to the high temperature conditions, the target genes of the differentially expressed microRNAs were primarily involved in metabolism adjustments. Our results will contribute to a better understanding of the regulatory roles of exosomes in sea cucumber, and provide insights into the functions of sea cucumber exosome-shuttled microRNAs against environmental stresses exacerbated by global warming.
关键词	Thermal stress Stress response Defense mechanism Extracellular vesicles MicroRNA Echinoderm
DOI	10.1016/j.ecoenv.2022.114419
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China ; Postdoctoral Innova-tive Talents Support Program of Shandong Province ; Major Scientific and Technological Innovation Projects in Shandong Province ; Shandong Provincial Natural Science Foundation ; Special Research Assistant Project of Chinese Academy of Sciences ; Qingdao Postdoctoral Applied Research Project ; Youth Innovation Promotion Association CAS ; [42106106] ; [42030408] ; [SDBX2020006] ; [2019JZZY010812] ; [ZR2021QD013] ; [2020170] ; [2019209]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000906446700001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181421
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China 8.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Huo, Da,Su, Fang,Yang, Hongsheng,et al. Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,249:10.
APA	Huo, Da,Su, Fang,Yang, Hongsheng,&Sun, Lina.(2023).Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,249,10.
MLA	Huo, Da,et al."Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 249(2023):10.