Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress

doi:10.3390/w15010063

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress
	Zhuo, Jintao 1,2,3; Wang, Hong 1,2,3; Du, Yifei 1,4; Shi, Mengmeng 1,5; Huan, Li1,3 ; Wang, Guangce 1,3
	2023
发表期刊	WATER
卷号	15 期号:1 页码:13
通讯作者	Huan, Li(huanli@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Since 2007, Ulva prolifera, a green macroalgal species that blooms periodically in the Yellow Sea of China, has caused the world's biggest green tide, which induced harmful ecological impacts and economic losses. When the alga is subject to prolonged exposure to air, it suffers abiotic stresses. To explore the physiological and molecular mechanisms of salt stress, the transcriptome data of U. prolifera at different salinities (30, 50, and 90 psu) were obtained by high-throughput sequencing using the Illumina HiSeq platform, and photosynthetic physiological parameters were also measured. The results showed that a total of 89,626 unigenes were obtained after de novo assembly, of which 60,441 unigenes were annotated in the databases (NR, NT, KO, SWISS-PROT, PFAM, GO, and KOG). GO functional enrichment analysis revealed that the enrichment of differentially expressed genes (DEGs) was mainly in cellular, cell, and binding processes. KEGG metabolic pathway enrichment analysis showed that the most frequently enriched pathways of DEGs included glycolysis, pyruvate metabolism, peroxisome, and fatty acid biosynthesis. In addition, resistance-associated proteins, such as heat-shock proteins, microtubule-associated proteins, ubiquitin-associated proteins, abscisic-acid-signaling-pathway-associated proteins, and antioxidant-related proteins are upregulated under salt stress. Genes associated with photosynthesis and carbon fixation pathways are also upregulated, accompanied by an increase in photosynthetic oxygen release rates. These findings provide a basis for understanding the molecular mechanisms of the response of U. prolifera to salinity change, thus providing a theoretical basis for the analysis of the green tide outbreak mechanism.
关键词	Ulva prolifera salt stress transcriptome photosynthetic physiological activities
DOI	10.3390/w15010063
收录类别	SCI
语种	英语
资助项目	Jiangsu Marine Science and Technology Innovation Project[JSZRHYKJ202206] ; China Agriculture Research System of MOF and MARA[CARS-50] ; National Natural Science Foundation of China[41976097]
WOS研究方向	Environmental Sciences & Ecology ; Water Resources
WOS类目	Environmental Sciences ; Water Resources
WOS记录号	WOS:000908936300001
出版者	MDPI
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181403
专题	实验海洋生物学重点实验室
通讯作者	Huan, Li; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Ocean Univ China, Coll Marine Life Sci, Qingdao 266100, Peoples R China 5.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhuo, Jintao,Wang, Hong,Du, Yifei,et al. Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress[J]. WATER,2023,15(1):13.
APA	Zhuo, Jintao,Wang, Hong,Du, Yifei,Shi, Mengmeng,Huan, Li,&Wang, Guangce.(2023).Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress.WATER,15(1),13.
MLA	Zhuo, Jintao,et al."Transcriptomic Analysis of Ulva prolifera in Response to Salt Stress".WATER 15.1(2023):13.