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Comparative study on the gonadal development in the diploid and artificially induced triploid olive flounder Paralichthys olivaceus
Wu, Qiaowan1,2,3,4; Song, Zongcheng5; Wang, Lijuan1,2; Wu, Zhihao1,2,7; Zou, Congcong1,2,3; Shu, Chang1,2,3; Liang, Shaoshuai1,2; Wang, Wenxiang1,2,3; Sun, Yuanyuan1,2; Yue, Xinlu4; Peng, Qinghua1,2,3,6; You, Feng1,2,7
2023-02-25
Source PublicationAQUACULTURE
ISSN0044-8486
Volume565Pages:15
Corresponding AuthorWu, Zhihao(wuzhihao@qdio.ac.cn) ; You, Feng(youfeng@qdio.ac.cn)
AbstractArtificial induction and breeding of triploids has been widely performed in fish due to their sterility or low fecundity. However, detailed studies on the gonadal development status, gamete formation, fertilization ability, and molecular mechanism of the induced triploids in marine fish are limited. Olive flounder Paralichthys olivaceus is an important mariculture fish in China, Japan, and Korea. In this study, by observing the gonadal development of the artificially induced triploid and control diploid flounder, the histological structure, sex hormone level, and transcriptome profiles were compared. The fecundity of the male triploid fish was also evaluated. The results showed that the development of the gonads of the triploids was severely inhibited. Apoptosis occurred in both male and female triploid gonads, which may lead to inhibition of germ cell development. At 24 months post hatch (mph), the contents of testosterone (T) and estradiol (E2) in the gonads of the female triploid flounder were lower than those of the diploid ones (P < 0.05), and the contents of E2 in the testes of the male triploid flounder were higher than those of the diploid ones (P < 0.05). Although the triploid flounder could produce sperm, the sperm presented different sizes of the head, deletion of the mitochondrion, and short tail under the scanning electron microscope. Only a few spermatozoa were slightly active. The ploidy of the spermatozoa included haploid, haploid -diploid, triploid, 4-5n aneuploidy, and even higher ploidy. By using sperm from the triploids with the control eggs, very few abnormal embryos and larvae were obtained. Transcriptome data at 18 and 24 mph showed that most of the significantly changed differentially expressed genes between the diploids and triploids were related to cell division and proliferation, energy and metabolism, fertility, and apoptosis. In the triploid testis, many genes related to spermatogenesis, such as mns1, dnah1, and dnah5, were down-regulated; while in the triploid ovary, genes related to testicular development, such as amh, amhr2, and wnt4, were up -regulated, which also provided molecular evidence for the testicular-like gonads observed in the histology of the triploid ovary. These results may help explain the developmental retardation and low fecundity in the gonads of triploid fish that are important for the future application of culture.
KeywordFlounder Triploid Gonadal development Transcriptome profile Sperm
DOI10.1016/j.aquaculture.2022.739106
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Founda-tion of China ; National Key Research and Devel-opment Program of China ; Key Research and Development Programs of Shandong province ; National Marine Genetic Resource Center ; [31772834] ; [2018YFD0900202] ; [2017GHY15115] ; [2021LZGC029] ; [2022TZXD004]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000901253800005
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/181388
Collection实验海洋生物学重点实验室
Corresponding AuthorWu, Zhihao; You, Feng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Zibo Vocat Inst, Zibo 255314, Peoples R China
5.Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China
6.Bingzhou Marine Dev & Res Inst, Binzhou 256600, Peoples R China
7.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Qiaowan,Song, Zongcheng,Wang, Lijuan,et al. Comparative study on the gonadal development in the diploid and artificially induced triploid olive flounder Paralichthys olivaceus[J]. AQUACULTURE,2023,565:15.
APA Wu, Qiaowan.,Song, Zongcheng.,Wang, Lijuan.,Wu, Zhihao.,Zou, Congcong.,...&You, Feng.(2023).Comparative study on the gonadal development in the diploid and artificially induced triploid olive flounder Paralichthys olivaceus.AQUACULTURE,565,15.
MLA Wu, Qiaowan,et al."Comparative study on the gonadal development in the diploid and artificially induced triploid olive flounder Paralichthys olivaceus".AQUACULTURE 565(2023):15.
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