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Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements
Li, Fengting1,2,3; Yue, Caixia1,4; Deng, Yunyan1,2,3; Tang, Ying Zhong1,2,3
2022-12-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号23期号:23页码:16
通讯作者Deng, Yunyan(yunyandeng@qdio.ac.cn) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
摘要Similar to the seeds of higher plants, resting cysts, a non-motile, benthic, and dormant stage in the life history of many dinoflagellate species, play vital roles via germination in the seasonal dynamics and particularly the initiation of harmful algal blooms (HABs) of dinoflagellates. It is thus crucial for resting cysts to balance between the energetic catabolism for viability maintenance and the energy preservation for germination during their dormancy. Despite this importance, studies on how resting cysts of dinoflagellates accomplish energetic metabolism in marine sediment have been virtually absent. In this study, using the cosmopolitan HABs-causing species Scrippsiella acuminata as a representative, we measured the transcriptional activity of the most efficient pathway of the energy catabolism tricarboxylic acid (TCA) cycle, cell viability (via neutral red staining), and the cellular ATP content of resting cysts under a set of mock conditions in marine sediments (e.g., 4 degrees C, darkness, and anoxia) for a maximum period of one year. Based on the correlation analyses among the expression levels of genes, cyst viability, and ATP content, we revealed that the TCA cycle was still a crucial pathway of energetic catabolism for resting cysts under aerobic conditions, and its expression was elevated at higher temperatures, light irradiation, and the early stage of dormancy. Under anaerobic conditions, however, the TCA cycle pathway ceased expression in resting cysts, as also supported by ATP measurements. Our results have laid a cornerstone for the comprehensive revelation of the energetic metabolism and biochemical processes of dormancy of resting cysts in marine sediments.
关键词ATP content energetic metabolism resting cyst Scrippsiella acuminata tricarboxylic acid cycle viability
DOI10.3390/ijms232315033
收录类别SCI
语种英语
资助项目Key Deployment Project of Centre for Ocean Mega - Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[42176207] ; Marine Samp;T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号WOS:000897362700001
出版者MDPI
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/181080
专题海洋生态与环境科学重点实验室
通讯作者Deng, Yunyan; Tang, Ying Zhong
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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GB/T 7714
Li, Fengting,Yue, Caixia,Deng, Yunyan,et al. Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(23):16.
APA Li, Fengting,Yue, Caixia,Deng, Yunyan,&Tang, Ying Zhong.(2022).Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(23),16.
MLA Li, Fengting,et al."Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.23(2022):16.
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