| Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills | |
He, Wanying1,2,3; Wang, Minxiao3,4,5; Li, Mengna3,4,5; Zhong, Zhaoshan4,5 ; Chen, Hao4,5; Xi, Shichuan1,2; Luan, Zhendong1,2,3; Li, Chaolun6; Zhang, Xin1,2,3
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| 2022-12-05 | |
| 发表期刊 | FRONTIERS IN MARINE SCIENCE
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| 卷号 | 9页码:12 |
| 通讯作者 | Li, Chaolun(lcl@qdio.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn) |
| 摘要 | Confocal Raman microscopy (CRM) is a powerful tool for biological research, which can provide information regarding the composition and distribution of biomolecules in an in situ, label-free, non-destructive manner and with high spatial resolution. Sample preservation is often an unavoidable step, especially for symbiotic deep-sea samples. Moreover, protocols for the preservation of samples for CRM have not been established and specific effects of different preservation methods on biomolecules have not been studied for relevant samples. In this study, we used deep-sea mussel Gigantidas platifrons, an ideal model in the study of deep-sea symbiosis and investigated the effect of four common preservation methods on the results of CRM imaging and signals. The methods included snap-freeze (SF), SF followed by rapid fixation in methanol (SF-MeOH), 2.5% glutaraldehyde and 2% paraformaldehyde fixation (SF-GP), and 4% paraformaldehyde and alcohol fixation (PS-PA). The results of this study indicate that SF was the most effective method for the comprehensive analysis of the biomolecular composition although the sectioning success rate was relatively low. Moreover, SF-MeOH was found to be effective when SF is not sufficient in obtaining good morphology in sections, or when the effect of chemical bonding on the composition of biomolecules upon SF-MeOH can be neglected. Finally, SF-GP and PS-PA were found to be the most effective methods considering the overall morphological observation. However, they were less suitable for metabolic studies. We believe our results can provide guidance for further studies of Raman on symbiotic deep-sea biological samples. It is of great importance for the wide application of Raman technique. |
| 关键词 | confocal Raman microscopy symbiotic deep-sea mussel preservation methods Raman imaging Raman signals |
| DOI | 10.3389/fmars.2022.1077595 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of Chinese Academy of Sciences[XDA22050102] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA19060402] ; Key project of Ocean Research Center, Chinese Academy of Sciences[COMS2020J03] ; National Natural Science Foundation of China[41822604] ; National Natural Science Foundation of China[42076091] ; Young Taishan Scholars Program[tsqn201909158] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| WOS类目 | Environmental Sciences ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000899661800001 |
| 出版者 | FRONTIERS MEDIA SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/181077 |
| 专题 | 海洋地质与环境重点实验室 海洋生态与环境科学重点实验室 |
| 通讯作者 | Li, Chaolun; Zhang, Xin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Chinese Acad Sci Key Lab Marine Geol & Environm, Ctr Deep Sea Res, Qingdao, Peoples R China 2.Pilot Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Chinese Acad Sci CAS Key Lab Marine Ecol & Environ, Ctr Deep Sea Res, Qingdao, Peoples R China 5.Lab Marine Ecol & Environm Sci, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 6.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | He, Wanying,Wang, Minxiao,Li, Mengna,et al. Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12. |
| APA | He, Wanying.,Wang, Minxiao.,Li, Mengna.,Zhong, Zhaoshan.,Chen, Hao.,...&Zhang, Xin.(2022).Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills.FRONTIERS IN MARINE SCIENCE,9,12. |
| MLA | He, Wanying,et al."Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills".FRONTIERS IN MARINE SCIENCE 9(2022):12. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| fmars-09-1077595.pdf（4373KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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