Fourier Transform Infrared Spectrometry Detection of Phaeodactylum tricornutum Biomacromolecules in Response to Environmental Changes

doi:10.1021/acsomega.2c05933

	Fourier Transform Infrared Spectrometry Detection of Phaeodactylum tricornutum Biomacromolecules in Response to Environmental Changes
	Han, Xiaotian 1,2,3,4; Zhao, Ting 1,2; He, Liyan 2,3; Jia, Yanfen 1,4; Yu, Ren-cheng 1,2,3
	2022-12-19
发表期刊	ACS OMEGA
ISSN	2470-1343
页码	7
通讯作者	Han, Xiaotian(xthan@qdio.ac.cn)
摘要	Environmental factors play an important role in the lipid, protein, and carbohydrate compositions of microalgae, wherein temperature and light are key influencing factors. Fourier transform infrared (FTIR) spectrometry was used in this study to detect biomacromolecules in Phaeodactylum tricornutum cells under different temperatures (10, 15, 20, and 25 ??C) and different illumination conditions (1000, 2000, 3000, and 4000 lx) to study the corresponding changes in lipid, protein, and carbohydrate contents. Results indicate that the biomacromolecule content at different temperatures has different patterns. Specifically, the patterns at 15 and 25 ??C are similar to each other and the contents accumulate with extended culture time. However, the pattern at 20 ??C is different. The carbohydrate and protein contents peaked during the early stage of the exponential phase, whereas lipid accumulation lagged behind the former two, peaking during the middle of the culture stage and then decreasing. Lipid content was analyzed by transmission electron microscopy (TEM), which revealed that the highest lipid content was observed at 15 ??C. Results also show that all of the lipid, protein, and carbohydrate contents in cells were the highest when the illumination was at 2000 lx and that the contents decreased with increasing illumination. By using FTIR, less samples were needed as compared to the traditional chemical quantitative detection methods. Moreover, the relative content changes of various biomacromolecules during the growth of P. tricornutum could be accurately determined by a single detection, thereby providing a new technique for the further study of metabolic mechanisms.
DOI	10.1021/acsomega.2c05933
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000903615800001
出版者	AMER CHEMICAL SOC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181041
专题	海洋生态与环境科学重点实验室
通讯作者	Han, Xiaotian
作者单位	1.Chinese Acad Sci, Inst Oceanol, Changjiang River Estuary Ecosystem Res Stn, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol Environm Sci, Qingdao 266071, Peoples R China 4.China Univ Geosciences, Sch Ocean Sci, Beijing 100083, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Han, Xiaotian,Zhao, Ting,He, Liyan,et al. Fourier Transform Infrared Spectrometry Detection of Phaeodactylum tricornutum Biomacromolecules in Response to Environmental Changes[J]. ACS OMEGA,2022:7.
APA	Han, Xiaotian,Zhao, Ting,He, Liyan,Jia, Yanfen,&Yu, Ren-cheng.(2022).Fourier Transform Infrared Spectrometry Detection of Phaeodactylum tricornutum Biomacromolecules in Response to Environmental Changes.ACS OMEGA,7.
MLA	Han, Xiaotian,et al."Fourier Transform Infrared Spectrometry Detection of Phaeodactylum tricornutum Biomacromolecules in Response to Environmental Changes".ACS OMEGA (2022):7.