Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks | |
Wang, Yingwen1,2,3; Zhang, Dun1,2,3; Zeng, Yan1,3; Sun, Yan1,3; Qi, Peng1,2,3 | |
2022-12-01 | |
发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL |
卷号 | 372页码:8 |
通讯作者 | Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn) |
摘要 | Paper-based sensors are particularly appealing for on-site detection owing to the avoidance of expensive and complex instruments. A dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase (HAase) release from aptamer-capped metal-organic frameworks (MOFs) was designed for on-site detection. The aptamercapped MOFs (HAase@MOF) were fabricated using UiO-67-NH2 as a nanocarrier for HAase and the doublestranded DNA (dsDNA) as a gatekeeper. The dsDNA structure of HAase@MOF was unlocked to release HAase in the presence of the target; thus, HAase hydrolyzed the hyaluronic acid hydrogel, changing the viscosity and acidity. The reaction solutions were pipetted onto the pH paper strips and the changes in the diffusion distance and the color signal were used to quantify the biotarget concentration. In this context, cytochrome c (cyt c) was selected as the model biotarget. The proposed sensor exhibited excellent specificity and reproducibility for detection of cyt c in human serum and bacterial biofilms. Overall, this study provides a low-cost, rapid workflow, and simple method for biotarget detection with dual-readout signal. Notably, other biotargets could be detected via directly changing the capping dsDNA structure of the MOFs. Thus, the dual-readout viscosity flow sensor possesses great promising in developing commercial products for on-site detection and sensing. |
关键词 | Dual-readout viscosity flow sensor Aptamer-capped MOF Biotarget triggered release Cytochrome c Hyaluronic acid hydrogel |
DOI | 10.1016/j.snb.2022.132637 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[JC2020090] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF01] ; Shandong Provincial Marine Science and Technology Projects |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
WOS记录号 | WOS:000888763800006 |
出版者 | ELSEVIER SCIENCE SA |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/180995 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Zhang, Dun; Qi, Peng |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,372:8. |
APA | Wang, Yingwen,Zhang, Dun,Zeng, Yan,Sun, Yan,&Qi, Peng.(2022).A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks.SENSORS AND ACTUATORS B-CHEMICAL,372,8. |
MLA | Wang, Yingwen,et al."A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks".SENSORS AND ACTUATORS B-CHEMICAL 372(2022):8. |
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