Unravelling the characterization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

doi:10.1111/are.16062

IOCAS-IR

	Unravelling the characterization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia
	Ni, Qian 1; Wang, Di 1; Xie, Lingli 1; Ge, Hongxing 1,2,3; Dong, Zhiguo 1,2,4
	2022-09-01
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
页码	9
通讯作者	Ge, Hongxing(hongxinggeliu@163.com) ; Dong, Zhiguo(dzg2004@163.com)
摘要	Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF-1 alpha sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open-reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF-1 alpha protein (744 amino acids) has the conserved HLH-PAS domain, which contained one N-terminal basic helix-loop-helix (bHLH) domain and two Per-ARNT-Sim A/B (PAS) domains, followed by PAS-associated C-terminal (PAC) domain. HIF-1 alpha is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF-1 alpha was significantly up-regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH-PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF-1 alpha gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.
关键词	antioxidant enzymes clone Cyclina sinensis HIF-1 alpha hypoxia
DOI	10.1111/are.16062
收录类别	SCI
语种	英语
资助项目	National key research and development projects[2020YFD0900203-02]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000848478300001
出版者	WILEY
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180907
专题	中国科学院海洋研究所
通讯作者	Ge, Hongxing; Dong, Zhiguo
作者单位	1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Jiangsu, Peoples R China 2.Jiangsu Inst Marine Resources Dev, Lianyungang, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lianyungang, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ni, Qian,Wang, Di,Xie, Lingli,et al. Unravelling the characterization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia[J]. AQUACULTURE RESEARCH,2022:9.
APA	Ni, Qian,Wang, Di,Xie, Lingli,Ge, Hongxing,&Dong, Zhiguo.(2022).Unravelling the characterization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia.AQUACULTURE RESEARCH,9.
MLA	Ni, Qian,et al."Unravelling the characterization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia".AQUACULTURE RESEARCH (2022):9.