Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis

doi:10.1016/j.aquaculture.2022.738624

IOCAS-IR > 实验海洋生物学重点实验室

	Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis
	Zhou, Shuhong 1,3; Jiang, Keyong 1; Liu, Mei 4; Wang, Baojie 1; Wang, Lei 1,2,4
	2022-12-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	561 页码:9
通讯作者	Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
摘要	The redclaw crayfish (Cherax quadricarinatus) is being farmed on an increasing scale in various tropical and subtropical regions. The most distinctive feature of the adult male redclaw crayfish is the red cuticle patch on the lateral cuticle of the chelae. It is an important indicator of the health status, reproduction, and resistance to predators of this species, but little is known about its functional properties. In this study, we used redclaw crayfish (45.35 +/- 16.74 g) as experimental subjects. Histological comparison of the red cuticle patch on male redclaw crayfish chelae with the cuticle on female redclaw crayfish chelae revealed that the red cuticle patch lacked exocuticle. High performance liquid chromatography analysis demonstrated that the concentration of free astaxanthin in the red cuticle patch was much higher than that in the chelae of female redclaw crayfish. Further analysis of transcriptome data from epithelial tissue of the chelae revealed that genes related to chela structure were highly expressed in the red cuticle patch of males. Immunity-related genes were not specifically expressed in the red cuticle patch, and immune-and growth-related genes that require calcium ion for activation were down-regulated in the patch. Our results indicated that regulation of genes related to astaxanthin binding was not a single up-or down-regulation mode. This study demonstrated structural differences in the chela cuticle between male and female redclaw crayfish and revealed the molecular basis for the functional properties of the red cuticle patch.
关键词	Cherax quadricarinatus Chela Red cuticle patch Histology Transcriptome
DOI	10.1016/j.aquaculture.2022.738624
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFD0900401] ; Yellow River Delta Industry Leading Talent Project
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000848337500009
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180832
专题	实验海洋生物学重点实验室
通讯作者	Wang, Baojie; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Marine Sci Res Inst Shandong Prov, Qingdao Aquat Organisms Qual Evaluat & Utilizat En, Qingdao 266104, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Shuhong,Jiang, Keyong,Liu, Mei,et al. Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis[J]. AQUACULTURE,2022,561:9.
APA	Zhou, Shuhong,Jiang, Keyong,Liu, Mei,Wang, Baojie,&Wang, Lei.(2022).Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis.AQUACULTURE,561,9.
MLA	Zhou, Shuhong,et al."Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis".AQUACULTURE 561(2022):9.

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