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Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis
Zhou, Shuhong1,3; Jiang, Keyong1; Liu, Mei4; Wang, Baojie1; Wang, Lei1,2,4
2022-12-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume561Pages:9
Corresponding AuthorWang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
AbstractThe redclaw crayfish (Cherax quadricarinatus) is being farmed on an increasing scale in various tropical and subtropical regions. The most distinctive feature of the adult male redclaw crayfish is the red cuticle patch on the lateral cuticle of the chelae. It is an important indicator of the health status, reproduction, and resistance to predators of this species, but little is known about its functional properties. In this study, we used redclaw crayfish (45.35 +/- 16.74 g) as experimental subjects. Histological comparison of the red cuticle patch on male redclaw crayfish chelae with the cuticle on female redclaw crayfish chelae revealed that the red cuticle patch lacked exocuticle. High performance liquid chromatography analysis demonstrated that the concentration of free astaxanthin in the red cuticle patch was much higher than that in the chelae of female redclaw crayfish. Further analysis of transcriptome data from epithelial tissue of the chelae revealed that genes related to chela structure were highly expressed in the red cuticle patch of males. Immunity-related genes were not specifically expressed in the red cuticle patch, and immune-and growth-related genes that require calcium ion for activation were down-regulated in the patch. Our results indicated that regulation of genes related to astaxanthin binding was not a single up-or down-regulation mode. This study demonstrated structural differences in the chela cuticle between male and female redclaw crayfish and revealed the molecular basis for the functional properties of the red cuticle patch.
KeywordCherax quadricarinatus Chela Red cuticle patch Histology Transcriptome
DOI10.1016/j.aquaculture.2022.738624
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2019YFD0900401] ; Yellow River Delta Industry Leading Talent Project
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000848337500009
PublisherELSEVIER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/180832
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Baojie; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Marine Sci Res Inst Shandong Prov, Qingdao Aquat Organisms Qual Evaluat & Utilizat En, Qingdao 266104, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Shuhong,Jiang, Keyong,Liu, Mei,et al. Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis[J]. AQUACULTURE,2022,561:9.
APA Zhou, Shuhong,Jiang, Keyong,Liu, Mei,Wang, Baojie,&Wang, Lei.(2022).Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis.AQUACULTURE,561,9.
MLA Zhou, Shuhong,et al."Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis".AQUACULTURE 561(2022):9.
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