Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

doi:10.1007/s00343-022-1358-0

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
	Jiao, Shuang 1; Tan, Xungang2,3 ; You, Feng 2,3; Zhang, Shujing 1; Pang, Qiuxiang 1
	2022-09-20
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	10
通讯作者	Jiao, Shuang(jiaoshuang@sdut.edu.cn) ; Tan, Xungang(tanx@qdio.ac.cn)
摘要	Insulin-like growth factors (IGFs) are key regulators of development and growth. Here, we characterized the igf2 gene from olive flounder (Paralichthys olivaceus) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder igf2 cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa), which spanned the complete signal peptide (47 aa), mature peptide (70 aa), and E domain (98 aa). In adult flounder, igf2 mRNA was detected in all selected tissues. In early development, igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region, floor plate, hypochord, otic vesicle, and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition, the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation, expression patterns, and biological activity of the igf2 in teleosts.
关键词	aquaculture flatfish igf2 proliferation
DOI	10.1007/s00343-022-1358-0
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31672636] ; Key Research and Development Program of Shandong Province, China[2019GHY112007]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000855632500003
出版者	SCIENCE PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180805
专题	实验海洋生物学重点实验室
通讯作者	Jiao, Shuang; Tan, Xungang
作者单位	1.Shandong Univ Technol, Sch Life Sci & Med, Antiaging & Regenerat Med Res Inst, Zibo 255000, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Jiao, Shuang,Tan, Xungang,You, Feng,et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2022:10.
APA	Jiao, Shuang,Tan, Xungang,You, Feng,Zhang, Shujing,&Pang, Qiuxiang.(2022).Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10.
MLA	Jiao, Shuang,et al."Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2022):10.