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A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation
Liu, Yuan1,2,3,4,6,7; Zhang, Ao1,2,5; Guo, Na1,2,5; Hao, Qiang1,2,5; Li, Fuhua1,2,3,4,6
2022-11-30
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号221页码:558-572
通讯作者Liu, Yuan(liuyuan@qdio.ac.cn)
摘要Ficolins, belonging to the fibrinogen-related protein superfamily, are important pattern recognition receptors in innate immunity. Here, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. The completed cDNA sequence of Ptficolin encoded a signal peptide, a coiled-coil region and a fibrinogen -like domain but without the typical collagen region of vertebrate ficolins. Ptficolin showed higher expression in stomach and hepatopancreas, and presented a time-dependent response after pathogen challenge and injury stimulation. The recombinant Ptficolin (rPtficolin) could bind to various PAMPs and microorganisms, and agglutinate microorganisms and rabbit erythrocytes in a Ca2+-dependent manner, with strong binding ability to N-acetyl sugars. Meanwhile, rPtficolin promoted the hemocyte phagocytosis and clearance activity of Vibrio, while Ptficolin knockdown impaired the bacterial phagocytosis and clearance ability, suggesting the opsonin activity of Ptficolin. Knockdown of Ptficolin could downregulate the transcription of most complement-like genes and AMPs, but enhance the expression of most proPO system-related genes and key genes of Toll, IMD and JNK pathways. Moreover, knockdown of Ptficolin led to the increased hemolymph clotting time and the decreased expression of clotting-related genes. Our results indicate that Ptficolin could recognize and eliminate invading pathogens, and might be a prominent component in hemolymph coagulation of crab.
关键词Ficolin Portunus trituberculatus Pattern recognition receptor Innate immunity Hemolymph clotting
DOI10.1016/j.ijbiomac.2022.09.041
收录类别SCI
语种英语
资助项目National Key R & D Program of China[2018YFD0901304] ; National Natural Science Foundation of China[41776159] ; China Agriculture Research System - 48[CARS -48]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号WOS:000859383200003
出版者ELSEVIER
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/180682
专题实验海洋生物学重点实验室
通讯作者Liu, Yuan
作者单位1.Chinese Acad Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Liu, Yuan,Zhang, Ao,Guo, Na,et al. A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,221:558-572.
APA Liu, Yuan,Zhang, Ao,Guo, Na,Hao, Qiang,&Li, Fuhua.(2022).A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,221,558-572.
MLA Liu, Yuan,et al."A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 221(2022):558-572.
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