Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations

doi:10.1093/plphys/kiac455

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations
	Zhou, Lu 1,2,3; Gao, Shan 1,2; Yang, Wenting 1,2; Wu, Songcui1,2 ; Huan, Li1,2 ; Xie, Xiujun 1,2; Wang, Xulei1,2 ; Lin, Senjie 4; Wang, Guangce 1,2
	2022-09-23
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
页码	20
通讯作者	Lin, Senjie(senjie.lin@uconn.edu) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Unlike in terrestrial and freshwater ecosystems, light fields in oceans fluctuate due to both horizontal current and vertical mixing. Diatoms thrive and dominate the phytoplankton community in these fluctuating light fields. However, the molecular mechanisms that regulate diatom acclimation and adaptation to light fluctuations are poorly understood. Here, we performed transcriptome sequencing, metabolome profiling, and C-13-tracer labeling on the model diatom Phaeodactylum tricornutum. The diatom acclimated to constant light conditions was transferred to six different light conditions, including constant light (CL5d), short-term (1 h) high light (sHL1h), and short-term (1 h) and long-term (5 days) mild or severe light fluctuation conditions (mFL1h, sFL1h, mFL5d, and sFL5d) that mimicked land and ocean light levels. We identified 2,673 transcripts (25% of the total expressed genes) expressed differentially under different fluctuating light regimes. We also identified 497 transcription factors, 228 not reported previously, which exhibited higher expression under light fluctuations, including 7 with a light-sensitive PAS domain (Per-period circadian protein, Arnt-aryl hydrocarbon receptor nuclear translocator protein, Sim-single-minded protein) and 10 predicted to regulate genes related to light-harvesting complex proteins. Our data showed that prolonged preconditioning in severe light fluctuation enhanced photosynthesis in P. tricornutum under this condition, as evidenced by increased oxygen evolution accompanied by the upregulation of Rubisco and light-harvesting proteins. Furthermore, severe light fluctuation diverted the metabolic flux of assimilated carbon preferentially toward fatty acid storage over sugar and protein. Our results suggest that P. tricornutum use a series of complex and different responsive schemes in photosynthesis and carbon metabolism to optimize their growth under mild and severe light fluctuations. These insights underscore the importance of using more intense conditions when investigating the resilience of phytoplankton to light fluctuations. Diatoms use a series of complex and different responsive schemes in photosynthesis and carbon metabolism to optimize their growth under mild and severe light fluctuations.
DOI	10.1093/plphys/kiac455
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; Chinese Academy of Sciences[KLEMB-DYS02] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Shandong Provincial RD Program[2018GHY115019] ; Distinguished Young Scientists Research Fund of the Key Laboratory of Experimental Marine Biology ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41976097]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000869808500001
出版者	OXFORD UNIV PRESS INC
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180633
专题	实验海洋生物学重点实验室
通讯作者	Lin, Senjie; Wang, Guangce
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhou, Lu,Gao, Shan,Yang, Wenting,et al. Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations[J]. PLANT PHYSIOLOGY,2022:20.
APA	Zhou, Lu.,Gao, Shan.,Yang, Wenting.,Wu, Songcui.,Huan, Li.,...&Wang, Guangce.(2022).Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations.PLANT PHYSIOLOGY,20.
MLA	Zhou, Lu,et al."Transcriptomic and metabolic signatures of diatom plasticity to light fluctuations".PLANT PHYSIOLOGY (2022):20.

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