Characterization of a novel beta-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates

doi:10.3389/fmicb.2022.972272

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of a novel beta-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates
	Gu, Xiaoqian 1,2; Zhao, Luying 1; Tan, Jiaojiao 2; Zhang, Qian 1; Fu, Liping 1; Li, Jiang 1
	2022-09-02
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	13 页码:12
通讯作者	Li, Jiang(lijiang@fio.org.cn)
摘要	An agarase gene (aga1904) that codes a protein with 640 amino acids was obtained from the metagenomic library of macroalgae-associated bacteria collected from King George Island, Antarctica. Gene aga1904 was expressed in Escherichia coli BL21 (DE3) and recombinant Aga1904 was purified by His Bind Purification kit. The optimal temperature and pH for the activity of Aga1904 were 50 degrees C and 6.0, respectively. Fe3+ and Cu2+ significantly inhibited the activity of Aga1904. The V-max and K-m values of recombinant Aga1904 were 108.70 mg/ml min and 6.51 mg/ml, respectively. The degradation products of Aga1904 against agarose substrate were mainly neoagarobiose, neoagarotetraose, and neoagarohexaose analyzed by thin layer chromatography. The cellular immunoassay of enzymatic hydrolysates was subsequently carried out, and the results showed that agaro-oligosaccharides dominated by neoagarobiose significantly inhibited key pro-inflammatory markers including, nitric oxide (NO), interleukins 6 (IL-6), and tumor necrosis factor alpha (TNF-alpha). This work provides a promising candidate for development recombinant industrial enzyme to prepare agaro-oligosaccharides, and paved up a new path for the exploitation of natural anti-inflammatory agent in the future.
关键词	metagenomic agarase biochemical characterization agaro-oligosaccharides anti-inflammatory
DOI	10.3389/fmicb.2022.972272
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000871635900001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180624
专题	实验海洋生物学重点实验室
通讯作者	Li, Jiang
作者单位	1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gu, Xiaoqian,Zhao, Luying,Tan, Jiaojiao,et al. Characterization of a novel beta-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates[J]. FRONTIERS IN MICROBIOLOGY,2022,13:12.
APA	Gu, Xiaoqian,Zhao, Luying,Tan, Jiaojiao,Zhang, Qian,Fu, Liping,&Li, Jiang.(2022).Characterization of a novel beta-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates.FRONTIERS IN MICROBIOLOGY,13,12.
MLA	Gu, Xiaoqian,et al."Characterization of a novel beta-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates".FRONTIERS IN MICROBIOLOGY 13(2022):12.