Deficiency of nitrogen but not phosphorus triggers the life cycle transition of the dinoflagellate Scrippsiella acuminata from vegetative growth to resting cyst formation

doi:10.1016/j.hal.2022.102312

	Deficiency of nitrogen but not phosphorus triggers the life cycle transition of the dinoflagellate Scrippsiella acuminata from vegetative growth to resting cyst formation
	Tang, Ying Zhong 1,2,3,4; Deng, Yunyan 1,2,3; Yue, Caixia 1,4; Chai, Zhaoyang 1,2,3; Shang, Lixia 1,2,3
	2022-10-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	118 页码:10
通讯作者	Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) ; Deng, Yunyan(yunyandeng@qdio.ac.cn)
摘要	Nitrogen (N) and phosphorus (P) are essential elements for algal growth. When N and P are deficient, dinoflagellates will take a series of measures to achieve population continuation including formation of resting cysts, an important ecological strategy of dinoflagellates that plays a key role in the initiation and termination of harmful algal blooms (HABs). How the deficiency of N and P affects algal growth and cyst formation has been investigated in some dinoflagellate species, but how it affects the life cycle transition in dinoflagellates has been poorly understood. In this study, we further explored the effect of N and P deficiency on the algal growth and resting cyst production in the cosmopolitan HABs-causing species Scrippsiella acuminata via refining the N and P concentration gradients. Further, we tracked the expression patterns of one CyclinB and one CDKI genes of S. acuminata at different growth stages under three deficiency concentrations (1/1000 dilutions of N, P, and both N and P). The results suggest that N deficiency always triggered the cyst formation but P deficiency mainly inhibited the vegetative growth instead of inducing cyst formation. We also observed the highest cyst production when S. acuminata was cultured in the f/2-Si medium that was a one-thousandth dilution of N and P (N similar to 0.882 mu M; P similar to 0.0362 mu M). Our results for the expressions of CyclinB and CDKI were well consistent with the results of algal growth and cyst formation at different deficiencies of N and P in terms of that higher expressions of these two genes were corresponding to higher rates of vegetative cell growth, while their expressions in resting cysts maintained to be moderate but significantly lower than that in fast-growing vegetative cells. Although we are still not sure whether the changing expressions of the two genes did regulate the transition of life cycle (i.e. cyst formation), or happened as parallels to the expressions of other truly regulating genes, our observations are surely inspirational for further investigations on the genetic regulation of life cycle transition in dinoflagellates. Our work will provide clues to probe the physiological and molecular mechanisms underlying the nutrient deficiency-induced alternation between life cycle stages in dinoflagellates.
关键词	Resting cyst Harmful algal blooms (HABs) Cell cycle CyclinB CDK1 Scrippsiella acuminata
DOI	10.1016/j.hal.2022.102312
收录类别	SCI
语种	英语
资助项目	Key Deployment Project of Center for Ocean Mega -Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[42176207] ; National Science Foundation of China[41976134]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000863319900006
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180585
专题	海洋生态与环境科学重点实验室
通讯作者	Tang, Ying Zhong; Deng, Yunyan
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Tang, Ying Zhong,Deng, Yunyan,Yue, Caixia,et al. Deficiency of nitrogen but not phosphorus triggers the life cycle transition of the dinoflagellate Scrippsiella acuminata from vegetative growth to resting cyst formation[J]. HARMFUL ALGAE,2022,118:10.
APA	Tang, Ying Zhong,Deng, Yunyan,Yue, Caixia,Chai, Zhaoyang,&Shang, Lixia.(2022).Deficiency of nitrogen but not phosphorus triggers the life cycle transition of the dinoflagellate Scrippsiella acuminata from vegetative growth to resting cyst formation.HARMFUL ALGAE,118,10.
MLA	Tang, Ying Zhong,et al."Deficiency of nitrogen but not phosphorus triggers the life cycle transition of the dinoflagellate Scrippsiella acuminata from vegetative growth to resting cyst formation".HARMFUL ALGAE 118(2022):10.

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