Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles | |
Xu, Chaojie1,2,3,4; Xing, Ronge1,2,3; Liu, Song1,2,3; Qin, Yukun1,2,3; Li, Kecheng1,2,3; Yu, Huahua1,2,3; Li, Pengcheng1,2,3 | |
2022-11-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
ISSN | 0141-8130 |
卷号 | 220页码:258-266 |
通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
摘要 | In this study, nanoparticles were prepared by using positively charged quaternized chitosan and negatively charged mucopolysaccharide such as chondroitin sulfate, heparin and hyaluronic acid. The nanoparticles have a stable nanostructure with particle size in 336.2-424.5 nm, potential in 18.5-31.1 mV and polydispersity index PDI of 0.172-0.335. Moreover, their encapsulation efficiency was 68.77 % and 64.89 %, and they have low endotoxin and good stability. It can significantly promote the expression of IL-6, TNF-alpha, and IL-1 beta of DCS cells. Moreover, the in vivo immune activity of heparin-quaternized chitosan-OVA nanoparticles against BALB/C mice was showed that, the nanoparticles could significantly promote the secretion of immunoglobulins in mice including IgG1 and IgG2. And nanoparticle also can promote the production of immune factors. Meanwhile, the expression of immune factor genes was also elevated. Furthermore, the results of tissue section experiments showed that the nanoparticles are safety of the body. |
关键词 | Mucopolysaccharide Quaternized chitosan Nanoparticles DCS cells Immunocompetence |
DOI | 10.1016/j.ijbiomac.2022.08.069 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Key deployment projects of Marine Science Research Center of Chinese Academy of Sciences[COMS2020J04] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
WOS记录号 | WOS:000862931600008 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/180570 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Xing, Ronge; Li, Pengcheng |
作者单位 | 1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,220:258-266. |
APA | Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2022).The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,220,258-266. |
MLA | Xu, Chaojie,et al."The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 220(2022):258-266. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
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