Genome of the sea anemone Exaiptasia pallida and transcriptome profiles during tentacle regeneration

doi:10.3389/fcell.2022.900321

IOCAS-IR > 实验海洋生物学重点实验室

	Genome of the sea anemone Exaiptasia pallida and transcriptome profiles during tentacle regeneration
	Shum, Cheryl W. Y.1; Nong, Wenyan 1; So, Wai Lok 1; Li, Yiqian 1; Qu, Zhe 1; Yip, Ho Yin 1; Swale, Thomas 2; Ang, Put O.3; Chan, King Ming 4; Chan, Ting Fung 5; Chu, Ka Hou 4,6; Chui, Apple P. Y.4; Lau, Kwok Fai 4; Ngai, Sai Ming 5; Xu, Fei 7; Hui, Jerome H. L.1
	2022-08-17
发表期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
ISSN	2296-634X
卷号	10 页码:13
通讯作者	Hui, Jerome H. L.(hui.jerome@gmail.com)
摘要	Cnidarians including sea anemones, corals, hydra, and jellyfishes are a group of animals well known for their regeneration capacity. However, how non-coding RNAs such as microRNAs (also known as miRNAs) contribute to cnidarian tissue regeneration is poorly understood. Here, we sequenced and assembled the genome of the sea anemone Exaiptasia pallida collected in Hong Kong waters. The assembled genome size of E. pallida is 229.21 Mb with a scaffold N50 of 10.58 Mb and BUSCO completeness of 91.1%, representing a significantly improved genome assembly of this species. The organization of ANTP-class homeobox genes in this anthozoan further supported the previous findings in jellyfishes, where most of these genes are mainly located on three scaffolds. Tentacles of E. pallida were excised, and both mRNA and miRNA were sequenced at 9 time points (0 h, 6 h, 12 h, 18 h, 1 day, 2, 3, 6, and 8 days) from regenerating tentacles. In addition to the Wnt signaling pathway and homeobox genes that are shown to be likely involved in tissue regeneration as in other cnidarians, we have shown that GLWamide neuropeptides, and for the first time sesquiterpenoid pathway genes could potentially be involved in the late phase of cnidarian tissue regeneration. The established sea anemone model will be useful for further investigation of biology and evolution in, and the effect of climate change on this important group of animals.
关键词	cnidarian sea anemone genome regeneration transcriptome microRNA
DOI	10.3389/fcell.2022.900321
收录类别	SCI
语种	英语
资助项目	TUYF Charitable Trust ; Hong Kong Research Grant Council Collaborative Research Grant[6904697] ; General Research Fund[C4015-20EF] ; PROCORE France/Hong Kong Joint Research Scheme[14100919] ; PROCORE France/Hong Kong Joint Research Scheme[14100420] ; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[F-CUHK402/18] ; Direct Grant of The Chinese University of Hong Kong[HKB L20200008] ; Chinese University of Hong Kong[4053489]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000873980300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180525
专题	实验海洋生物学重点实验室
通讯作者	Hui, Jerome H. L.
作者单位	1.Chinese Univ Hong Kong, Sch Life Sci, Simon FS Li Marine Sci Lab, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China 2.Dovetail Genom, Scotts Valley, CA USA 3.Chinese Univ Hong Kong, Inst Space & Earth Informat Sci, Hong Kong, Peoples R China 4.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China 5.Chinese Univ Hong Kong, Sch Life Sci, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China 6.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Shum, Cheryl W. Y.,Nong, Wenyan,So, Wai Lok,et al. Genome of the sea anemone Exaiptasia pallida and transcriptome profiles during tentacle regeneration[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2022,10:13.
APA	Shum, Cheryl W. Y..,Nong, Wenyan.,So, Wai Lok.,Li, Yiqian.,Qu, Zhe.,...&Hui, Jerome H. L..(2022).Genome of the sea anemone Exaiptasia pallida and transcriptome profiles during tentacle regeneration.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,13.
MLA	Shum, Cheryl W. Y.,et al."Genome of the sea anemone Exaiptasia pallida and transcriptome profiles during tentacle regeneration".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10(2022):13.