Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker

doi:10.3390/microorganisms10102028

	Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker
	Huang, Hailong 1,2,3; Xu, Qing 2,3,4; Song, Huiyin 2,3,4; Chen, Nansheng 2,3,4,5
	2022-10-01
发表期刊	MICROORGANISMS
卷号	10 期号:10 页码:14
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	Diatoms are responsible for approximately 40% of the global primary photosynthetic production and account for up to 20% of global carbon fixation. Coscinodiscus granii is a red tide forming species of the phylum Bacillariophyta that has been detected in a wide range of coastal regions, suggesting the possibility of the existence of high genetic diversity with differential adaptation. Common molecular markers including 18S rDNA, 16S rDNA, ITS, cox1, and rbcL do not provide sufficient resolution for distinguishing intra-species genetic diversity, hindering in-depth research on intra-species genetic diversity and their spatial and temporal dynamics. In this project, we aimed to develop molecular markers with high resolution and specificity for C. granii, attempting to identify different taxa of this species, which will set up a stage for subsequent functional assays. Comparative genomics analysis of the mtDNAs of C. granii strains identified a genomic region with high genomic variations, which was used to guide the development of a molecular marker with high resolution and high specificity. This new molecular marker, which was named cgmt1 (C. granii mitochondrial 1), was 376 bp in size and differentiated C. granii samples collected in coastal regions of China into three different clades. Preliminary analysis of field samples collected in various coastal regions in China revealed that C. granii clades were almost exclusively found in the Bohai Sea and the north Yellow Sea. This newly developed molecular marker cgmt1 could be used for tracking intra-species genetic diversity and biogeographic distribution of C. granii in different ecosystems.
关键词	red tide species Coscinodiscus granii mitochondrial genomes (mtDNAs) molecular marker genetic diversity
DOI	10.3390/microorganisms10102028
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of China[42176162] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan[19-3-2-16-zhc] ; Post-doctoral Program of Qingdao[E0KY061] ; Natural Science Foundation of Shandong Province, China[ZR2020QD095]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000873129200001
出版者	MDPI
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180474
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Huang, Hailong,Xu, Qing,Song, Huiyin,et al. Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker[J]. MICROORGANISMS,2022,10(10):14.
APA	Huang, Hailong,Xu, Qing,Song, Huiyin,&Chen, Nansheng.(2022).Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker.MICROORGANISMS,10(10),14.
MLA	Huang, Hailong,et al."Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker".MICROORGANISMS 10.10(2022):14.

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