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| A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms | |
Xu, Hang1,2,3,4 ; Yuan, Zihao1,2,3; Sun, Li1,2,3,4
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| 2022-11-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| Volume | 23Issue:21Pages:15 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) |
| Abstract | NK-lysin (NKL) is a family of antimicrobial proteins with an important role in innate and adaptive immunity. In this study, a non-canonical NK-lysin (NKLnc) was identified in the Japanese flounder (Paralichthys olivaceus), which shares low sequence identities (15.8-20.6%) with previously reported fish NKLs and was phylogenetically separated from the canonical NKLs in teleost. NKLnc expression was upregulated in flounder tissues during bacterial infection, and interference with NKLnc expression impaired the ability of flounder cells to eliminate invading bacteria. When expressed in Escherichia coli, NKLnc was detrimental to the host cells. P35, a peptide derived from the saposin B domain (SapB) of NKLnc, bound major bacterial surface molecules and killed both Gram-negative and Gram-positive bacteria by inflicting damage to bacterial cell structure and genomic DNA. The bactericidal activity, but not the bacteria-binding capacity, of P35 required the structural integrity of the alpha 2/3 helices in SapB. Furthermore, P35 induced the migration of flounder peripheral blood leukocytes, inhibited bacterial dissemination in fish tissues, and facilitated fish survival after bacterial challenge. Together our study reveals that NKLnc plays an important part in flounder immune defense, and that NKLnc peptide exerts an antimicrobial effect via multiple mechanisms by targeting both bacteria and fish cells. |
| Keyword | NK-lysin Paralichthys olivaceus antimicrobial peptide bactericidal |
| DOI | 10.3390/ijms232112722 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS ID | WOS:000881196400001 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/180417 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li |
| Affiliation | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Xu, Hang,Yuan, Zihao,Sun, Li. A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(21):15. |
| APA | Xu, Hang,Yuan, Zihao,&Sun, Li.(2022).A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(21),15. |
| MLA | Xu, Hang,et al."A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.21(2022):15. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| ijms-23-12722-v2.pdf（5362KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | View | |
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