A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms

doi:10.3390/ijms232112722

IOCAS-IR > 实验海洋生物学重点实验室

	A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms
	Xu, Hang1,2,3,4 ; Yuan, Zihao1,2,3 ; Sun, Li 1,2,3,4
	2022-11-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:21 页码:15
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	NK-lysin (NKL) is a family of antimicrobial proteins with an important role in innate and adaptive immunity. In this study, a non-canonical NK-lysin (NKLnc) was identified in the Japanese flounder (Paralichthys olivaceus), which shares low sequence identities (15.8-20.6%) with previously reported fish NKLs and was phylogenetically separated from the canonical NKLs in teleost. NKLnc expression was upregulated in flounder tissues during bacterial infection, and interference with NKLnc expression impaired the ability of flounder cells to eliminate invading bacteria. When expressed in Escherichia coli, NKLnc was detrimental to the host cells. P35, a peptide derived from the saposin B domain (SapB) of NKLnc, bound major bacterial surface molecules and killed both Gram-negative and Gram-positive bacteria by inflicting damage to bacterial cell structure and genomic DNA. The bactericidal activity, but not the bacteria-binding capacity, of P35 required the structural integrity of the alpha 2/3 helices in SapB. Furthermore, P35 induced the migration of flounder peripheral blood leukocytes, inhibited bacterial dissemination in fish tissues, and facilitated fish survival after bacterial challenge. Together our study reveals that NKLnc plays an important part in flounder immune defense, and that NKLnc peptide exerts an antimicrobial effect via multiple mechanisms by targeting both bacteria and fish cells.
关键词	NK-lysin Paralichthys olivaceus antimicrobial peptide bactericidal
DOI	10.3390/ijms232112722
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000881196400001
出版者	MDPI
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180417
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Xu, Hang,Yuan, Zihao,Sun, Li. A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(21):15.
APA	Xu, Hang,Yuan, Zihao,&Sun, Li.(2022).A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(21),15.
MLA	Xu, Hang,et al."A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.21(2022):15.

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ijms-23-12722-v2.pdf（5362KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览