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Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration
Shao, Zhizhuo1,2,3; Xie, Xiujun1,2; Liu, Xueying1,4; Zheng, Zhenbing1,2; Huan, Li1,2; Zhang, Baoyu1,2; Wang, Guangce1,2
2022-07-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume66Pages:9
Corresponding AuthorWang, Guangce(gcwang@qdio.ac.cn)
AbstractThere is no clear evidence of an operative xanthophyll cycle in species containing phycobilisomes, including red algae and cyanobacteria. In particular, intertidal red algae are subjected to adverse conditions such as high light (HL) stress periodically. Therefore, how red algae avert light-induced damage for photoprotection has attracted much interest. Photosynthetic oxygen evolution (POE) and photosystem II (PSII) activity analysis showed that Neopyropia yezoensis, an important economic red algae in the intertidal zone, possessed relatively high photosynthetic activity despite being under light intensity beyond the required of growth. However, such HL intensity has forced many algae to experience photoinhibition. Through systematic analysis, we found that gamma-type carbonic anhydrase-like1 (gamma CAL1) protein in mitochondria plays a key photoprotective role. We propose that the CO2 produced by photorespiration under HL is transformed into bicarbonate, through gamma CAL1, to prevent the escape of CO2. Bicarbonate is converted back into CO2, which is enriched near the Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), causing an increase in the partial pressure of CO2. To verify this result, we constructed gamma CAL1 overexpressing strains. It is interesting to discover the decreasing photorespiration flux in the transgenic strain, compared with the wild type (WT), is consistent with the findings reported in higher plants. Based on the above results, it is clear that promoting the efficiency of the Calvin cycle is an important mechanism for N. yezoensis to resist HL stress. During this process, sufficient CO2 can be supplied by photorespiration, where gamma CAL1 directly participates. Therefore, our research provides new evidence for revealing the strategies of phycobilisome-containing resurrection algae tolerating excess light energy.
KeywordNeopyropia yezoensis Photorespiration Gamma -type carbonic anhydrase-like1 Calvin cycle
DOI10.1016/j.algal.2022.102766
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41876163] ; National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41776150] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000880611400014
PublisherELSEVIER
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/180372
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Guangce
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Ocean Univ China OUC, Coll Marine Life Sci, Qingdao 266003, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shao, Zhizhuo,Xie, Xiujun,Liu, Xueying,et al. Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,66:9.
APA Shao, Zhizhuo.,Xie, Xiujun.,Liu, Xueying.,Zheng, Zhenbing.,Huan, Li.,...&Wang, Guangce.(2022).Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,66,9.
MLA Shao, Zhizhuo,et al."Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 66(2022):9.
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