Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration

doi:10.1016/j.algal.2022.102766

IOCAS-IR > 实验海洋生物学重点实验室

	Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration
	Shao, Zhizhuo 1,2,3; Xie, Xiujun 1,2; Liu, Xueying 1,4; Zheng, Zhenbing 1,2; Huan, Li1,2 ; Zhang, Baoyu 1,2; Wang, Guangce 1,2
	2022-07-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	66 页码:9
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	There is no clear evidence of an operative xanthophyll cycle in species containing phycobilisomes, including red algae and cyanobacteria. In particular, intertidal red algae are subjected to adverse conditions such as high light (HL) stress periodically. Therefore, how red algae avert light-induced damage for photoprotection has attracted much interest. Photosynthetic oxygen evolution (POE) and photosystem II (PSII) activity analysis showed that Neopyropia yezoensis, an important economic red algae in the intertidal zone, possessed relatively high photosynthetic activity despite being under light intensity beyond the required of growth. However, such HL intensity has forced many algae to experience photoinhibition. Through systematic analysis, we found that gamma-type carbonic anhydrase-like1 (gamma CAL1) protein in mitochondria plays a key photoprotective role. We propose that the CO2 produced by photorespiration under HL is transformed into bicarbonate, through gamma CAL1, to prevent the escape of CO2. Bicarbonate is converted back into CO2, which is enriched near the Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), causing an increase in the partial pressure of CO2. To verify this result, we constructed gamma CAL1 overexpressing strains. It is interesting to discover the decreasing photorespiration flux in the transgenic strain, compared with the wild type (WT), is consistent with the findings reported in higher plants. Based on the above results, it is clear that promoting the efficiency of the Calvin cycle is an important mechanism for N. yezoensis to resist HL stress. During this process, sufficient CO2 can be supplied by photorespiration, where gamma CAL1 directly participates. Therefore, our research provides new evidence for revealing the strategies of phycobilisome-containing resurrection algae tolerating excess light energy.
关键词	Neopyropia yezoensis Photorespiration Gamma -type carbonic anhydrase-like1 Calvin cycle
DOI	10.1016/j.algal.2022.102766
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41876163] ; National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41776150] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000880611400014
出版者	ELSEVIER
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180372
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Ocean Univ China OUC, Coll Marine Life Sci, Qingdao 266003, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shao, Zhizhuo,Xie, Xiujun,Liu, Xueying,et al. Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,66:9.
APA	Shao, Zhizhuo.,Xie, Xiujun.,Liu, Xueying.,Zheng, Zhenbing.,Huan, Li.,...&Wang, Guangce.(2022).Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,66,9.
MLA	Shao, Zhizhuo,et al."Overexpression of mitochondrial?CAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 66(2022):9.