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Examination of the regulation of energy metabolism, antioxidant response, and ammonia detoxification in hard clam, Mercenaria mercenaria, under hypersalinity stress
Zhou, Cong1,2,3,4,5,6; Xu, Li1,7; Song, Hao1,2,3,4,6; Feng, Jie1,2,3,4,6; Hu, Zhi1,2,3,4,5,6; Yang, Mei-jie1,2,3,4,5,6; Shi, Pu1,2,3,4,5,6; Li, Youg-Reh8; Guo, Yong-Jun8; Li, Hai-Zhou9; Zhang, Tao1,2,3,4,6,10
2023-01-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume563Pages:11
Corresponding AuthorZhang, Tao(tzhang@qdio.ac.cn)
AbstractWith global warming on the rise, hypersalinity events are occurring with increasing frequency due to accelerated evaporation rates of seawater. Hypersalinity affects the distribution, growth, and physiological processes of aquatic organisms, especially for osmoconforming bivalves that lack complete means of osmoregulation. In this way, knowledge concerning comprehensive metabolic regulation is limited in hypersalinity-stressed bivalves. The hard clam, Mercenaria mercenaria , is an important pond-farmed bivalve in China, with an annual output value of 150 million USD. This species can be farmed in salt pans because it is highly resistant to hypersalinity. In this study, we investigated the comprehensive pattern of metabolic regulation in hard clams under hypersalinity stress. We identified 979 metabolites in the gills of hard clams, 295 of which were differentially accumulated after different periods of exposure to hypersalinity (8 h, 1 day, and 5 days), compared with the control. Critical hypersalinity-responsive metabolites, including acyl carnitine, oxidized lipids, and several antioxidants were identified using K-means analysis. During hypersalinity exposure, the activities of hexokinase and pyruvate ki-nase, and the pyruvic acid content significantly increased, suggesting that glycolysis was enhanced. Alanine and glycine were identified as key free amino acids involved in osmoregulaion. After 5 days of hypersalinity expo-sure, the accumulation of alanine and lactic acid marked the initiation of anaerobic glycolysis. The up-regulation of amino acid catabolism and inhibition of ammonia excretion led to ammonia accumulation in the gills. Glutamine synthetase and glutamate dehydrogenase activities significantly increased to reduce ammonia toxicity. Many antioxidant metabolites, such as allantoin, cinnamic acid, and trigonelline, were dramatically up -regulated to relieve hypersalinity-induced oxidative stress. The accumulation of lysophosphatides suggested that cell membranes damaged by prolonged oxidative stress and/or hyperosmosis were dissolved by phospholipase A1. This process helped to maintain overall cellular homeostasis. Potential biomarkers of hypersalinity stress in hard clams were identified. These results will facilitate the assessment of the physiological status of hard clam in aquaculture and provide novel insights into the metabolic regulatory mechanisms of bivalves under hypersalinity stress.
KeywordHypersalinity Osmoconformer Metabolomics Ammonia detoxification Oxidative stress
DOI10.1016/j.aquaculture.2022.738916
Indexed BySCI
Language英语
Funding ProjectTianjin science and technology commission project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; earmarked fund for CARS[CARS-49] ; Shandong Province Agricultural Major Applied Technology Innovation Project[SF1405303301] ; Taishan Industrial Leading Talents Project[LJNY201704] ; Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Young Elite Scientists Sponsorship Program by CAST[2021QNRC001]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000877475300004
PublisherELSEVIER
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/180333
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Tao
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
7.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aqua ecol & Aquaculture, Tianjin 300384, Peoples R China
9.Shandong Fu Han Ocean Scitech Co Ltd, Haiyang 265100, Peoples R China
10.7 Nanhai Rd, Qingdao, Shandong, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Cong,Xu, Li,Song, Hao,et al. Examination of the regulation of energy metabolism, antioxidant response, and ammonia detoxification in hard clam, Mercenaria mercenaria, under hypersalinity stress[J]. AQUACULTURE,2023,563:11.
APA Zhou, Cong.,Xu, Li.,Song, Hao.,Feng, Jie.,Hu, Zhi.,...&Zhang, Tao.(2023).Examination of the regulation of energy metabolism, antioxidant response, and ammonia detoxification in hard clam, Mercenaria mercenaria, under hypersalinity stress.AQUACULTURE,563,11.
MLA Zhou, Cong,et al."Examination of the regulation of energy metabolism, antioxidant response, and ammonia detoxification in hard clam, Mercenaria mercenaria, under hypersalinity stress".AQUACULTURE 563(2023):11.
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