Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation

doi:10.1016/j.memsci.2022.121132

	Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation
	Lin, Yanna 1; Xu, Yulong 1; Xing, Yanzhi 1; Liu, Nazhen 2; Chen, Xuwei 1
	2023-01-05
发表期刊	JOURNAL OF MEMBRANE SCIENCE
ISSN	0376-7388
卷号	665 页码:13
通讯作者	Liu, Nazhen(liunazhen@qdio.ac.cn) ; Chen, Xuwei(chenxuwei@mail.neu.edu.cn)
摘要	Membrane separation is an efficient technique for the separation and extraction of biomolecules from complex matrices. Herein, a thrombin aptamer-functionalized temperature-responsive smart gating metal-organic framework (MOF) membrane was fabricated by three-dimensional ordered macroporous ZIF-8 (3DOM ZIF-8) and copolymer of sodium alginate grafted with poly(N-isopropylacrylamide) (SA-PNIPAM) for thrombin (TB) isolation. The SA-PNIPAM on the exterior surface of 3DOM MOFs endows the membrane with favorable protein penetration selectivity via temperature-controlled size-screening effect, and the aptamer functionalized on the interior macropore provides excellent thrombin capturing specificity. The separation factor of the smart gating membrane ranges from 13.88 to 49.22 for mixtures of TB with other protein species, and TB enrichment factor is high up to 95.97 under a TB/HSA mass ratio of 1:1000. The aptamer functionalized smart gating membrane is applied to the selective isolation of TB from the human serum. Fibrin production tests demonstrate that the biological activity of the isolated TB is well maintained and suitable for ensuing applications. Moreover, this 3DOM MOF-based smarting gating membrane exhibits high fouling-resistance performance.
关键词	Three-dimensional ordered macroporous metal -organic framework Membrane separation Thrombin Aptamer Size screening
DOI	10.1016/j.memsci.2022.121132
收录类别	SCI
语种	英语
资助项目	LiaoNing Science and Technology Development Foundation Guided by Central Government[2022JH6/100100024] ; Fundamental Research Funds for the Cen- tral Universities[N2005027] ; National Natural Science Foundation of China[22074011]
WOS研究方向	Engineering ; Polymer Science
WOS类目	Engineering, Chemical ; Polymer Science
WOS记录号	WOS:000885059500002
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180302
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Liu, Nazhen; Chen, Xuwei
作者单位	1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Box 332, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation[J]. JOURNAL OF MEMBRANE SCIENCE,2023,665:13.
APA	Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2023).Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation.JOURNAL OF MEMBRANE SCIENCE,665,13.
MLA	Lin, Yanna,et al."Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation".JOURNAL OF MEMBRANE SCIENCE 665(2023):13.

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