Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction

doi:10.1016/j.snb.2022.132749

	Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction
	Zhou, Yanan 1,2,3,4; Zeng, Yan1,2 ; Zhang, Dun 1,2; Qi, Peng1,2 ; Wang, Yingwen 1,2,3,4; Xin, Yue 1,2,3,4; Sun, Yan 1,2
	2023
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	374 页码:7
通讯作者	Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
摘要	We have constructed a simple, enzyme-free and label-free detection method for bacterial DNA. First, bacterial DNA (T) is designed as a trigger to make the designed metastable hairpin probes P1, P2, P3 (carrying DNA silver nanoclusters sequences) autonomously cross-open to achieve catalytic self-assembly, inducing a three-way DNA junction formation through the catalyzed hairpin self-assembly (CHA) mode. Then, G-rich sequences are intro-duced to form DNA silver nanoclusters G quadruplex (DNA-AgNCs-G quadruplex), and a strong fluorescence signal can be collected under the action of silver nitrate and sodium borohydride solutions. Through the fluo-rescent reporter of DNA silver nanoclusters (DNA-AgNCs) and the amplification of CHA, we successfully detected target DNA at concentrations as low as 19.95 fM. Compared with the constructed acyclic system, the sensitivity of this cyclic system is improved about three orders of magnitude. Moreover, this recycling system achieves good selectivity for mismatched target DNA and excellent recovery rate in practical samples. Therefore, bacterial DNA analysis based on target-aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction is successfully developed, promising its great application in biological sensing.
关键词	Bacterial DNA DNA silver nanocluster Three-way DNA junction
DOI	10.1016/j.snb.2022.132749
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Nantong Scientific Plan Foundation[JC2020090] ; Nantong Scientific Plan Foundation[JC2021054] ; Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF01] ; Shandong Provincial Marine Science and Technology Projects
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000882029800004
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180281
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zeng, Yan; Zhang, Dun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Yanan,Zeng, Yan,Zhang, Dun,et al. Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,374:7.
APA	Zhou, Yanan.,Zeng, Yan.,Zhang, Dun.,Qi, Peng.,Wang, Yingwen.,...&Sun, Yan.(2023).Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction.SENSORS AND ACTUATORS B-CHEMICAL,374,7.
MLA	Zhou, Yanan,et al."Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction".SENSORS AND ACTUATORS B-CHEMICAL 374(2023):7.

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