Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance

doi:10.1186/s13068-022-02221-y

IOCAS-IR > 实验海洋生物学重点实验室

	Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance
	Gao, Shan 1,2; Zhou, Lu 1,2,3; Yang, Wenting 4; Wang, Lijun 1,2; Liu, Xuehua 1,2; Gong, Yingchun 5; Hu, Qiang 6,7; Wang, Guangce 1,2
	2022-11-26
发表期刊	BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
卷号	15 期号:1 页码:19
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Background The marine diatom Phaeodactylum tricornutum is a commercially viable species due to its bioactive substances and lipid productivity. Increasing attention has been paid to the isolation or genetic modification of species or strains with a rapid growth rate and large quantities of lipids. Furthermore, contamination of microzooplankton has been one of the major constraints in P. tricornutum large-scale cultivation, which adversely affects growth and greatly impedes the course of biomass production industrialization. Results Here, based on our previous transcriptomics of P. tricornutum, we found a novel gene (ID: 7202015, hereafter called Pt2015) which affects morphotype of P. tricornutum. Pt2015 protein is located in the plastid, which is highly homologous to part of the sequences of exosome component. The morphotype of the Pt2015 knockout strain (termed 2015KO) using CRISPR/Cas9 method is fusiform, but the Pt2015 overexpression strain (termed oeT) demonstrates a majority triradiate morphotype (approximately 95%) which is stable and has been cultured for more than 200 generations. In addition, the oeT strain demonstrated a similar growth rate to the WT and simultaneously accumulated larger lipids droplets that increased by approximately 30% compared to that of the WT. More importantly, the grazing rate of the amoebae cultured in the oeT strain significantly decreased in comparison with that cultured in WT, suggesting that the oeT can effectively avoid being eaten by microzooplankton. Conclusions Therefore, the oeT strain not only improves our understanding of morphotype conversion in diatoms but also demonstrates potential applications for large-scale cultivation of P. tricornutum.
关键词	Diatom Gene manipulation Lipid Morphotype Phaeodactylum tricornutum
DOI	10.1186/s13068-022-02221-y
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[42106157]
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000888808400001
出版者	BMC
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180244
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Peoples R China 6.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen 518055, Peoples R China 7.Chinese Acad Sci, Fac Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao, Shan,Zhou, Lu,Yang, Wenting,et al. Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance[J]. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,2022,15(1):19.
APA	Gao, Shan.,Zhou, Lu.,Yang, Wenting.,Wang, Lijun.,Liu, Xuehua.,...&Wang, Guangce.(2022).Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance.BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,15(1),19.
MLA	Gao, Shan,et al."Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance".BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 15.1(2022):19.

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