Enhanced resolution of marine viruses with violet side scatter

doi:10.1002/cyto.a.24674

	Enhanced resolution of marine viruses with violet side scatter
	Zhao, Yuan 1,2,3; Zhao, Yanchu 1,2,4; Zheng, Shan 2,3,5; Zhao, Li 1,2,3; Zhang, Wuchang 1,2,3; Xiao, Tian 1,2,3; Gregori, Gerald 6
	2022-08-05
发表期刊	CYTOMETRY PART A
ISSN	1552-4922
页码	9
通讯作者	Gregori, Gerald(gerald.gregori@univ-amu.fr)
摘要	Marine viruses make up an essential compartment of the marine ecosystem. They are the most abundant organisms and represent one of the biggest sources of unknown biodiversity. Viruses also have an important impact on bacterial and algal mortality in the ocean, and as such have a major influence on microbial diversity and biogeochemical cycling. However, little is known about the abundance and distribution patterns of viruses across the oceans and seas. Over the last 20 years, flow cytometry has been the technique of choice to detect and count the viral particles in natural samples. Nevertheless, due to their small size, the detection of marine viruses is still extremely challenging. In this article we describe how a new generation of flow cytometer which uses the side scatter (SSC) of violet photons from a 405 nm laser beam helps to improve the resolution for detecting marine viruses. To the best of our knowledge, this is the first report where virioplankton has been detected in aquatic samples using flow cytometry with a 405 nm violet SSC instead of a 488 nm blue SSC.
关键词	flow cytometry 405 nm side scatter sub-clusters SYBR Green I virus like particles
DOI	10.1002/cyto.a.24674
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42076139] ; Open Fund of Pilot National Laboratory for Marine Science and Technology (Qingdao)[QNLM20160RP0311] ; CNRS-NSFC Joint Research Projects Program[CNRS-272110] ; CNRS-NSFC Joint Research Projects Program[NSFC 41711530149] ; 2017-2019 Sino-French Cai Yuanpei Program ; International Research Project-Dynamics and Function of Marine Microorganisms (IRP-DYF2M): insight from physics and remote sensing, CNRS-CAS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemical Research Methods ; Cell Biology
WOS记录号	WOS:000836282700001
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179867
专题	海洋生态与环境科学重点实验室
通讯作者	Gregori, Gerald
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Marine Ecosyst Res Stn, Qingdao, Peoples R China 6.Aix Marseille Univ, Toulon Univ, Mediterranean Inst Oceanog UM110, CNRS,IRD, Marseille, France
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhao, Yuan,Zhao, Yanchu,Zheng, Shan,et al. Enhanced resolution of marine viruses with violet side scatter[J]. CYTOMETRY PART A,2022:9.
APA	Zhao, Yuan.,Zhao, Yanchu.,Zheng, Shan.,Zhao, Li.,Zhang, Wuchang.,...&Gregori, Gerald.(2022).Enhanced resolution of marine viruses with violet side scatter.CYTOMETRY PART A,9.
MLA	Zhao, Yuan,et al."Enhanced resolution of marine viruses with violet side scatter".CYTOMETRY PART A (2022):9.