Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)

doi:10.3390/genes13071262

IOCAS-IR > 实验海洋生物学重点实验室

	Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)
	Wu, Yanduo 1,2,3,4,5; Xiao, Yongshuang 1,2,3,4; Xiao, Zhizhong 1,2,3,4; Ma, Yuting 1,2,4,6; Zhao, Haixia1,2,4,5 ; Li, Jun 1,2,3,4
	2022-07-01
发表期刊	GENES
卷号	13 期号:7 页码:11
通讯作者	Li, Jun(junli@qdio.ac.cn)
摘要	The spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high ecological value, food value, and fishing value, and its growth has obvious sex dimorphism. The rapid identification of its sex is beneficial to the development of sex determination and breeding. In this study, the method of comparative genomics and PCR amplification was used to further establish a rapid detection method for the recombinant RhoGEF10 gene in O. punctatus, which can quickly, accurately, and efficiently identify the sex of the O. punctatus to be tested. The homologous comparison results of male and female individuals showed that the DNA fragment length of the RhoGEF10 gene on the X1 chromosome was 326 bp, and the DNA fragment length on the Y chromosome was 879 bp. Therefore, it can be concluded that there is an insert fragment of 553 bp on the Y chromosome. PCR amplification results showed that the two DNA fragments of 879 bp and 326 bp were amplified in the Y chromosome and X1 chromosome of the male O. punctatus (X1X2Y), respectively, and the 879 bp fragment was a unique marker fragment of the recombinant RhoGEF10 gene; The female O. punctatus (X1X1X2X2) only a single DNA fragment of 326 bp was amplified. At the same time, the inserted fragment of the male individual resulted in partial inactivation of the RhoGEF10 protein, which in turn resulted in a slowing of peripheral nerve conduction velocity and thinning of the myelin sheath in male O. punctatus. The method shortens the time for accurate identification of the O. punctatus RhoGEF10 gene recombination and improves the detection efficiency. It is of great significance and application value in the research of nerve conduction and myelin development, male and female sex identification, the preparation of high male seedlings, and family selection based on the RhoGEF10 gene in the O. punctatus.
关键词	Oplegnathus punctatus RhoGEF10 gene insertion deletion polymorphism sex molecular marker
DOI	10.3390/genes13071262
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05] ; CARS for Marine Fish Culture Industry[CARS-47] ; Major Agricultural Application Technology Innovation Project of Shandong Province[SD2019YY011] ; STS project[KFZD-SW106] ; STS project[ZSSD-019] ; STS project[2017T3017] ; STS project[KFJ-STS-QYZX-020] ; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Natural Science Foundation of China[31672672]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000833644600001
出版者	MDPI
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179815
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Sch Oceanog, Beijing 100049, Peoples R China 6.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wu, Yanduo,Xiao, Yongshuang,Xiao, Zhizhong,et al. Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)[J]. GENES,2022,13(7):11.
APA	Wu, Yanduo,Xiao, Yongshuang,Xiao, Zhizhong,Ma, Yuting,Zhao, Haixia,&Li, Jun.(2022).Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus).GENES,13(7),11.
MLA	Wu, Yanduo,et al."Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)".GENES 13.7(2022):11.