Transcriptome profiles of genes related to growth and virulence potential in Vibrio alginolyticus treated with modified clay

doi:10.1016/j.micres.2022.127095

	Transcriptome profiles of genes related to growth and virulence potential in Vibrio alginolyticus treated with modified clay
	Ding, Yu 1,2,3,4; Song, Xiuxian1,2,3,4 ; Yu, Zhiming1,2,3,4
	2022-09-01
发表期刊	MICROBIOLOGICAL RESEARCH
ISSN	0944-5013
卷号	262 页码:12
通讯作者	Song, Xiuxian(songxx@qdio.ac.cn)
摘要	Vibrio alginolyticus is a globally distributed opportunistic pathogen that causes different degrees of disease in various marine organisms, such as fish, shrimp and shellfish. At present, vibriosis caused by V. alginolyticus has a wide epidemic range and causes frequent outbreaks, resulting in substantial losses in aquaculture. According to previous studies, modified clay (MC) could effectively flocculate and reduce the density of Vibrio in water, but it is still unknown whether MC inhibits growth and how it affects virulence in bottom flocs. Here, we studied the response mechanism of V. alginolyticus in flocs treated with MC at the transcriptome level and verified the transcriptomic data combined with relevant physiological experiments and reverse transcription quantitative real-time PCR (RT-qPCR) for the first time. It was found that the morphology of Vibrio in the MC flocs changed, the membrane function was damaged, the antioxidant system was activated, and the material and energy metabolism also changed. In addition, MC could inhibit the expression of virulence factors of V. alginolyticus; for example, flagella, pilus, siderophores, quorum sensing, and other related genes were significantly downregulated. In general, MC effectively inhibited the growth of Vibrio and reduced its virulence potential in flocs, which could provide theoretical support for a new model of healthy aquaculture.
关键词	Vibrio alginolyticus Modified clay Transcriptome Virulence Growth
DOI	10.1016/j.micres.2022.127095
收录类别	SCI
语种	英语
资助项目	Key R & D project of Shandong Province[2019JZZY010808] ; Taishan Program of Shandong Province of 2019 ; National Natural Science Foundation of China[41976148]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000819418600004
出版者	ELSEVIER GMBH
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179653
专题	海洋生态与环境科学重点实验室
通讯作者	Song, Xiuxian
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Lab Marine Ecol & Environm Sci, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Ding, Yu,Song, Xiuxian,Yu, Zhiming. Transcriptome profiles of genes related to growth and virulence potential in Vibrio alginolyticus treated with modified clay[J]. MICROBIOLOGICAL RESEARCH,2022,262:12.
APA	Ding, Yu,Song, Xiuxian,&Yu, Zhiming.(2022).Transcriptome profiles of genes related to growth and virulence potential in Vibrio alginolyticus treated with modified clay.MICROBIOLOGICAL RESEARCH,262,12.
MLA	Ding, Yu,et al."Transcriptome profiles of genes related to growth and virulence potential in Vibrio alginolyticus treated with modified clay".MICROBIOLOGICAL RESEARCH 262(2022):12.