Establishment and preliminary study of electrophysiological techniques in a typical red tide species

doi:10.1016/j.scitotenv.2022.156698

	Establishment and preliminary study of electrophysiological techniques in a typical red tide species
	Wang, Zhongshi 1,2,3,4; Yu, Zhiming1,2,3,4,6 ; He, Liyan 1,2,4; Zhu, Jianan 1,2,4; Liu, Lidong 5; Song, Xiuxian1,2,3,4
	2022-09-20
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	840 页码:11
通讯作者	Yu, Zhiming(zyu@qdio.ac.cn)
摘要	Electrophysiology studies the electrical properties of cells and tissues including bioelectrical signals and membrane ion channel activities. As an important means to reveal ion channel related physiological functions and the underlying mechanisms, electrophysiological techniques have been widely used in studies of animals, higher plants and algae that are closely related to higher plants. However, few electrophysiological studies have been carried out in red tide organisms, especially in dinoflagellates, which is mainly due to the complex surface structure of dinoflagellate amphiesma. In this study, the surface amphiesma of Alexandrium pacificum, a typical red tide species, was removed by centrifugation, low-temperature treatment and enzymatic treatment. In all three treatments, low-temperature treatment with 4 C for 2 h had high ecdysis rate and high fixation rate, and the treated cells were easy to puncture, so low temperature treatment was used as a preprocessing treatment for subsequent current recording. Acquired protoplasts of A. pacificum were identified by calcofluor fluorescence and immobilized by poly-lysine. A modified "puncture " single-electrode voltage-clamp recording was first applied to dinoflagellates, and voltage-gated currents, which had the characteristics of outward K+ current and inward Cl(- )current, were recorded and confirmed by ion replacement, indicating the voltage-gated currents were mixed. This method can be used as a technical basis for the electrophysiological study of dinoflagellates and provides a new perspective for the study of stress tolerance, red tide succession, and the regulation of physiological function of dinoflagellates.
关键词	Electrophysiological technique Dinoflagellate Protoplast Voltage-clamp Alexandrium pacificum
DOI	10.1016/j.scitotenv.2022.156698
收录类别	SCI
语种	英语
资助项目	Institute of Oceanology, Chinese Academy of Sciences ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001-1] ; Taishan Program of Shandong Province of 2019 ; National Natural Science Foundation of China[42006120] ; Special Research Assistant Program of Chinese Academy of Sciences
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000816997600005
出版者	ELSEVIER
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179635
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Zhiming
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Univ British Columbia, Djavad Mowafaghian Ctr Brian Hlth, Dept Med, Vancouver, BC, Canada 6.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Zhongshi,Yu, Zhiming,He, Liyan,et al. Establishment and preliminary study of electrophysiological techniques in a typical red tide species[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,840:11.
APA	Wang, Zhongshi,Yu, Zhiming,He, Liyan,Zhu, Jianan,Liu, Lidong,&Song, Xiuxian.(2022).Establishment and preliminary study of electrophysiological techniques in a typical red tide species.SCIENCE OF THE TOTAL ENVIRONMENT,840,11.
MLA	Wang, Zhongshi,et al."Establishment and preliminary study of electrophysiological techniques in a typical red tide species".SCIENCE OF THE TOTAL ENVIRONMENT 840(2022):11.