IOCAS-IR
UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus
Wang, Min1; Du, Guicai2; Fang, Junna3; Wang, Linsong4; Guo, Qunqun2; Zhang, Tingting2; Li, Ronggui2
2022-05-31
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号13页码:15
通讯作者Li, Ronggui(lrg@qdu.edu.cn)
摘要Pine wilt disease (PWD) caused by Bursaphelenchus xylophilus is considered a major threat to pine forests worldwide. Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic compounds with sugars and play crucial roles in the detoxification and homeostatic processes in all living organisms. We investigated the molecular characteristics and biological functions of the gene UGT440A1 that encodes UGTs in B. xylophilus. The in situ hybridization results indicated that UGT440A1 is expressed in all developmental stages of B. xylophilus, particularly in the head, intestine, and hypodermis of the second-stage of juveniles (J2), third-stage of juveniles (J3) and fourth-stage of juveniles (J4) females and in almost the whole body of J4 males and adults. Recombinant UGT440A1 was observed mainly in the inclusion bodies, and the enzyme activity assay revealed that UGT440A1 could catalyze the glycosylation reaction of two types of flavonols (kaempferol and quercetin). RNA interference (RNAi) of UGT440A1 suppressed motility, feeding, and reproduction of B. xylophilus. Furthermore, UGT440A1 knockdown caused a delay in the development of PWD symptoms in the pine seedlings inoculated with the nematodes. These results suggest that UGT440A1 is involved in the pathogenic process of B. xylophilus and the information may facilitate a better understanding of the molecular mechanism of PWD.
关键词Bursaphelenchus xylophilus UDP-glycosyltransferase motility reproduction pathogenicity
DOI10.3389/fpls.2022.862594
收录类别SCI
语种英语
资助项目Key Programs for Research and Development of Shandong Province, China[2016GNC110024] ; National Natural Science Foundation of China[31070575] ; National Natural Science Foundation of China[31901313]
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000810931300001
出版者FRONTIERS MEDIA SA
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/179534
专题中国科学院海洋研究所
通讯作者Li, Ronggui
作者单位1.Qingdao Univ, Med Coll, Qingdao, Peoples R China
2.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China
3.Qingdao JiMo Peoples Hosp, Qingdao, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
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Wang, Min,Du, Guicai,Fang, Junna,et al. UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus[J]. FRONTIERS IN PLANT SCIENCE,2022,13:15.
APA Wang, Min.,Du, Guicai.,Fang, Junna.,Wang, Linsong.,Guo, Qunqun.,...&Li, Ronggui.(2022).UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus.FRONTIERS IN PLANT SCIENCE,13,15.
MLA Wang, Min,et al."UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus".FRONTIERS IN PLANT SCIENCE 13(2022):15.
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