<p>High cobalt exposure facilitates bioactive exopolysaccharides production with a novel molecular structure in Botryococcus braunii</p>

doi:10.1016/j.cej.2022.136294

IOCAS-IR

	High cobalt exposure facilitates bioactive exopolysaccharides production with a novel molecular structure in Botryococcus braunii
	Cheng, Pengfei 1; Chang, Ting 1; Wang, Chun1 ; Yao, Changhong 2; Zhou, Chengxu 1; Liu, Tianzhong 3; Wang, Guangce 4; Yan, Xiaojun 5; Ruan, Roger 6
	2022-08-15
发表期刊	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
卷号	442 页码:12
通讯作者	Yao, Changhong(yaochanghong@scu.edu.cn) ; Yan, Xiaojun(yanxiaojun@nbu.edu.cn) ; Ruan, Roger(ruanx001@umn.edu)
摘要	The promising unicellular colonial Botryococcus braunii can secrete large amounts of bioactive exopoly-saccharides. Our previous study revealed Botryococcus could tolerate high-concentrations of metallic cobalt, and extracellular hydrocarbons increased simultaneously. However, the characteristics of the extracellular poly-saccharides (EPS) closely related to extracellular hydrocarbons is still unclear.& nbsp;This research focuses on the ef-fects of cobalt exposure on the production, structure, and bioactivity of exopolysaccharides from colonial Botryococcus, aiming to provide a theoretical clue on the potential contribution of EPS to the cobalt tolerance ability in this alga for cobalt bioremediation from the perspective of structure-bioactivity relationship. The results showed that high cobalt treatment (4.5 mg/L) exerted little influence on the growth of the alga, or the total carbohydrate content obtained, compared to the algae without cobalt treatment. EPSs of B. braunii SAG 30.81, under normal condition (EPS-Co) was mainly composed of glucose, mannose, fucose, galactose, rhamnose, and xylose, but also with the presence of sulfate substitutions, which was unique among the EPSs previously reported in this alga. Cobalt treatment resulted in a remarkable decline of rhamnose, and an increase of ribose and sulfate content, with more esterified uronic acids in the EPS (EPS + Co). EPS + Co, overall, had a larger but more compact and branched, spherical-like EPSs, with more ordered (helix) chains relative to EPS-Co. EPS + Co exhibited stronger antioxidant and antibacterial activity than EPS-Co, which could be attributed to the changed structural characteristics, such as the decrease of Rha, Fuc, and Gal and an increase of sulfate substitutions, uronic acids and Ara, more branched structure, and more ordered conformation. The enhanced antioxidant activity could be contributed to the alga tolerance to cobalt exposure, which was benefitted by cobalt bioremediation.
关键词	Botryococcus braunii Cobalt exposure Exopolysaccharide Chemical structure Bioactivity
DOI	10.1016/j.cej.2022.136294
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFA0903003] ; National Natural Science Foundation of China[32170369] ; Natural Science Foundation of Zhejiang Province[LZJWY22B070001] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2019NO3] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[MRUKF2021003] ; University of Minnesota MnDrive Environment Program[MNE12] ; University of Minnesota Center for Biorefining
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:000797852300004
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179414
专题	中国科学院海洋研究所
通讯作者	Yao, Changhong; Yan, Xiaojun; Ruan, Roger
作者单位	1.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Sichuan Univ, Sch Chem Engn, Dept Pharmaceut & Biol Engn, Chengdu 610065, Sichuan, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 5.Ningbo Univ, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Zhejiang, Peoples R China 6.Univ Minnesota Twin Cities, Ctr Biorefining, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
推荐引用方式 GB/T 7714	Cheng, Pengfei,Chang, Ting,Wang, Chun,et al. High cobalt exposure facilitates bioactive exopolysaccharides production with a novel molecular structure in Botryococcus braunii [J]. CHEMICAL ENGINEERING JOURNAL,2022,442:12.
APA	Cheng, Pengfei.,Chang, Ting.,Wang, Chun.,Yao, Changhong.,Zhou, Chengxu.,...&Ruan, Roger.(2022). High cobalt exposure facilitates bioactive exopolysaccharides production with a novel molecular structure in Botryococcus braunii .CHEMICAL ENGINEERING JOURNAL,442,12.
MLA	Cheng, Pengfei,et al." High cobalt exposure facilitates bioactive exopolysaccharides production with a novel molecular structure in Botryococcus braunii ".CHEMICAL ENGINEERING JOURNAL 442(2022):12.

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