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Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production
Liu, Minlu1; Krock, Bernd2; Yu, Rencheng3; Leaw, Chui Pin4; Lim, Po Teen4; Ding, Guangmao5; Wang, Na1; Zheng, Jing1; Gu, Haifeng1,6,7
2022-06-01
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume115Pages:14
Corresponding AuthorGu, Haifeng(guhaifeng@tio.org.cn)
AbstractThe bloom-forming dinophyte Alexandrium minutum comprises biogeographic inferred, global and Pacific clades with both toxic and nontoxic strains reported. A. minutum has a wide distribution in the Western Pacific, but to date only a few strains have available DNA sequences. To fully understand its genetic diversity, sampling was undertaken from the Yellow Sea, the East and South China Sea, and five strains of A. minutum and two strains of its sister species, A. tamutum, were established. Their morphology was examined by light and scanning electron microscopy. In addition, sequences were obtained from both large subunit (LSU) ribosomal DNA and/or internal transcribed spacer (ITS) region. Strains of A. minutum are morphologically indistinguishable, characterized by a smaller cell size and a narrow sixth precingular plate. In contrast, A. tamutum has a wider sixth precingular plate. High nucleotide divergences of LSU (D1-D3) rDNA and ITS were revealed amongst strains of A. minutum (10% and 25%, respectively), and A. tamutum (3% and 13%, respectively). Molecular phylogenies based on LSU rDNA and ITS revealed three ribotypes (B-D) of A. minutum, and two ribotypes of A. tamutum in the Western Pacific. Seasonal sampling in the East China Sea to detect A. minutum using the DNA metabarcoding targeting ITS1 region was also performed. Our results showed that the ribotypes B and C of A. minutum co-occurred in the water. Paralytic shellfish toxin (PSTs) of all seven strains was analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). PSTs were detected only in A. minutum ribotypes B and C with predominance of gonyautoxins 1/4. Our results suggest high diversity and risk potential of this toxic species in this region.
KeywordCryptic species Dinoflagellates Harmful algal bloom (HAB) Metabarcoding Paralytic shellfish toxins (PSTs)
DOI10.1016/j.hal.2022.102238
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[42030404] ; National Key Research and Development Program of China[2019YFE0124700] ; Fund Project for Marine and Fishery Protection and Development in Fujian Province[FZJZ-2021-1] ; Ministry of Higher Education of Malaysia HICOE ; [LRGS/1/2020/UMT/01/1/3]
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000798742200001
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/179384
Collection海洋生态与环境科学重点实验室
Corresponding AuthorGu, Haifeng
Affiliation1.Minist Nat Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China
2.Helmholtz Zent Polar & Meeresforschung, Alfred Wegener Inst, Handelshafen 12, D-27570 Bremerhaven, Germany
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Univ Malaya, Inst Ocean & Earth Sci, Bachok Marine Res Stn, Bachok 16310, Kelantan, Malaysia
5.Fishery Resources Monitoring Ctr Fujian Prov, Fuzhou 350003, Peoples R China
6.Minist Nat Resources, Key Lab Marine Ecol Conservat & Restorat, Xiamen 361005, Peoples R China
7.Fujian Prov Key Lab Marine Ecol Conservat & Restor, Xiamen 361005, Peoples R China
Recommended Citation
GB/T 7714
Liu, Minlu,Krock, Bernd,Yu, Rencheng,et al. Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production[J]. HARMFUL ALGAE,2022,115:14.
APA Liu, Minlu.,Krock, Bernd.,Yu, Rencheng.,Leaw, Chui Pin.,Lim, Po Teen.,...&Gu, Haifeng.(2022).Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production.HARMFUL ALGAE,115,14.
MLA Liu, Minlu,et al."Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production".HARMFUL ALGAE 115(2022):14.
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